LOCUS       sequence-129069-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a fusion of mCherry with the membrane located DH domain 
            (1-347) of p63RhoGEF in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   van Unen J, Reinhard NR, Yin T, Wu YI, Postma M, Gadella TW, 
            Goedhart J
  TITLE     Plasma membrane restricted RhoGEF activity is sufficient for 
            RhoA-mediated actin polymerization.
  JOURNAL   Sci Rep. 2015 Oct 5;5:14693. doi: 10.1038/srep14693.
  PUBMED    26435194
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-5"; volume: "5:14693. doi"; pages: " 
            10.1038/srep14693"
COMMENT     Sequence Label: pmCherry-C1-pmDH
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      623..632
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
       61 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cagttatcta
      121 gatccggtgg atcccgggcc cgcggtacct tagccctcaa atccccgcaa tctccccagc
      181 gtcatcatat cgttgcagcg cttgggcaca aagcacatga cctccacagc ttgctctagg
      241 tctgcagtat ccatcccagc tctattgtaa tacttgagaa aatccttgag cagcagctgg
      301 tatttcatga tccgctgcac aggtttgatg aggaggtcgt tcagctgcag gcggtgcccc
      361 agctgctgcc ggagctcctc aaagtagctg tccccaaact ctgacaccac atgctctgac
      421 ttgggcttat tctgacagta caccacatac atatgcagcc ggcgctcgtg tttgatgaat
      481 agctgagcca gccaatcagg atctttcaga caccgttgta gctcttgcaa gaaatagtct
      541 cggtgccact catagatttg ctggatattc ccaaacacaa tcctgtcacg gcctcgaaga
      601 ctctcgggga ccccctgagc agccatggtg gccatataac cctccacaat ctgccccaag
      661 tcgtccacgt acattttctc tgtttctacc agttcactca ggacatacat actcctttcc
      721 agagccttct tcttctgttc ctcctcactc tcaggg
//
