LOCUS       sequence-129070-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a fusion of mCherry with the membrane located DH domain 
            (1-347) of p63RhoGEF in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   van Unen J, Reinhard NR, Yin T, Wu YI, Postma M, Gadella TW, 
            Goedhart J
  TITLE     Plasma membrane restricted RhoGEF activity is sufficient for 
            RhoA-mediated actin polymerization.
  JOURNAL   Sci Rep. 2015 Oct 5;5:14693. doi: 10.1038/srep14693.
  PUBMED    26435194
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-5"; volume: "5:14693. doi"; pages: " 
            10.1038/srep14693"
COMMENT     Sequence Label: pmCherry-C1-pmDH
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             50..757
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(195..213)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     463..482
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             761..844
                     /codon_start=1
                     /product="palmitoylation signal consisting of the first 29 
                     amino acids of p63RhoGEF (Goedhart et al., 2013)"
                     /label=palmitoylation signal
                     /translation="RGGHKGGRCACPRVIRKVLAKCGCCFAR"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggtgagcaa
       61 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
      121 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
      181 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
      241 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
      301 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
      361 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
      421 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
      481 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
      541 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
      601 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
      661 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
      721 ccgccactcc accggcggca tggacgagct gtacaagtcc cggggggggc acaaaggggg
      781 tcgctgtgcc tgtccccgtg tgatccgaaa agtgctggca aaatgcggct gctgcttcgc
      841 ccggggggg
//
