LOCUS       sequence-129071-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a fusion of mCherry with FKBP12 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   van Unen J, Reinhard NR, Yin T, Wu YI, Postma M, Gadella TW, 
            Goedhart J
  TITLE     Plasma membrane restricted RhoGEF activity is sufficient for 
            RhoA-mediated actin polymerization.
  JOURNAL   Sci Rep. 2015 Oct 5;5:14693. doi: 10.1038/srep14693.
  PUBMED    26435194
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-5"; volume: "5:14693. doi"; pages: " 
            10.1038/srep14693"
COMMENT     Sequence Label: pmCherry-FKBP12-C1
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             47..751
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELY"
     primer_bind     complement(192..210)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     460..479
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg
       61 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
      121 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
      181 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
      241 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
      301 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
      361 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
      421 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
      481 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
      541 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
      601 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
      661 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
      721 ccactccacc ggcggcatgg acgagctgta cagagcggcg atgggagtgc aggtggaaac
      781 catctcccca ggagacgggc gcaccttccc caagcgcggc cagacctgcg tggtgcacta
      841 caccgggatg cttgaagatg gaaagaaatt tgat
//
