LOCUS       sequence-129079-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_539_2xUAS_dCP
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    240..264
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(329..353)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    400..491
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(432..451)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    505..553
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gcgccgggcc tttctttatg tttttggcgt cttccatggt ggctttacca acagtaccgg
       61 aatgccgttt ggtatgcgtc ttgtgattca aagttggctt attcaaagga tattaacgaa
      121 ggcagcggca cgtctgctcg gctcgaacat tcattcacaa ctgatgctct cagccacccc
      181 gcgccctttt ataccgctgc gctcgatccc cgggcgagct cgatcgcaga tcttgatata
      241 ccactttgta caagaaagct gggtcctcgg aggacagtac tccgacctgc aggcatgcaa
      301 gcttccgctc ggaggacagt actccggaag cctgcttttt tgtacaaact tgtttgatat
      361 cctcgaggct agcgagctca ggtaccggcc agttaggcca gagaaatgtt ctggcacctg
      421 cacttgcact ggggacagcc tattttgcta gtttgttttg tttcgttttg ttttgatgga
      481 gagcgtatgt tagtactatc gattcacaca aaaaaccaac acacagatgt aatgaaaata
      541 aagatatttt attgcggcct gtccaatacc tcccgtacct taatattact tacttatcct
      601 tgagagacgt actagtaacc ctgataaatg cttcaataat attgaaaaag gacgagtatg
      661 agcatccaac attttcgtgt cgcactcatt cccttctttg cggcattttg cttgcctgtt
      721 tttgcacacc ccgaaacgct ggtgaaagta aaagatgctg aagatcaact gggtgcaaga
      781 gtgggctata tcgaactgga tctcaatagc ggcaagatcc ttgagtcttt
//
