LOCUS       sequence-129080-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_539_2xUAS_dCP
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    54..78
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(143..167)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      364..373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(436..454)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tttctctggc ctaactggcc ggtacctgag ctcgctagcc tcgaggatat caaacaagtt
       61 tgtacaaaaa agcaggcttc cggagtactg tcctccgagc ggaagcttgc atgcctgcag
      121 gtcggagtac tgtcctccga ggacccagct ttcttgtaca aagtggtata tcaagatctg
      181 cgatcgagct cgcccgggga tcgagcgcag cggtataaaa gggcgcgggg tggctgagag
      241 catcagttgt gaatgaatgt tcgagccgag cagacgtgcc gctgccttcg ttaatatcct
      301 ttgaataagc caactttgaa tcacaagacg cataccaaac ggcattccgg tactgttggt
      361 aaagccacca tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg
      421 gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct
      481 ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc
      541 gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga
      601 atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt
      661 atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt
      721 atgggcattt cgcagcctac cgtggtgttc gtttccaaaa aggggttgca aaaaattttg
      781 aacgtgcaaa aaaagctccc aatcatcc
//
