LOCUS       sequence-129090-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to 
            clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Nguyen NT, Topkar VV, Zheng Z, 
            Joung JK
  TITLE     Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 
            by modifying PAM recognition.
  JOURNAL   Nat Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404.
  PUBMED    26524662
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404."
COMMENT     Sequence Label: BPK2660
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(258..506)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(316..335)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(486..506)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        613..717
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
       61 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt catatgtaga
      121 gaggtacctc gagcggccca agcttaaaaa aatctcgcca acaagttgac gagataaaca
      181 cggcattttg ccttgtttta gtagattctg taattttcat tacagagtac taaaaccgag
      241 acgcattaat cgtctcgggt gtttcgtcct ttccacaaga tatataaagc caagaaatcg
      301 aaatactttc aagttacggt aagcatatga tagtccattt taaaacataa ttttaaaact
      361 gcaaactacc caagaaatta ttactttcta cgtcacgtat tttgtactaa tatctttgtg
      421 tttacagtca aattaattct aattatctct ctaacagcct tgtatcgtat atgcaaatat
      481 gaaggaatca tgggaaatag gccctcttcc tgcccgacct tggtaccgga tccagtcgac
      541 tgaattggtt cctttaaagc ctgctttttt gtacagctag cgacgtcagg tggcactttt
      601 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      661 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      721 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
      781 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
      841 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
      901 ga
//
