LOCUS       sequence-129098-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of CENP-I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKM343
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..68
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             81..125
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 agtccggagg ggatcggtgg agctccaccg gtggcggccg ctctagagag aatctttatt
       61 ttcagggcgc cgccaaattc aaagaaaccg ctgctgctaa attcgaacgc cagcacatgg
      121 acagcggagg tggaggtact agtctcgaga tgagcccaca gaaacgtgta aaaaatgtcc
      181 aggcccagaa ccgtaccagt caaggcagta gcagcttcca aacaaccctg tcagcatgga
      241 aagtgaaaca ggacccgagc aacagcaaaa acatctctaa acatggccag aataacccag
      301 ttggcgacta tgagcacgcc gatgatcaag cagaagagga cgccctgcaa atggcggtag
      361 gctatttcga aaaaggccct atcaaagcca gtcagaacaa agacaaaacg ctggagaaac
      421 acctgaaaac cgtggagaac gtggcatgga aaaatggtct ggcgagcgaa gaaatcgaca
      481 tcctgctgaa tattgccctg agcggcaaat ttggtaatgc cgtgaatacc cgtatcctga
      541 aatgtatgat cccggcaacc gtgattagtg aagattccgt cgtgaaagcg gtgtcatggc
      601 tgtgtgttgg caaatgttcc ggtagcacga aagttctgtt ctatcgctgg ctggttgcca
      661 tgttcgattt tatcgaccgt aaagagcaga tcaacctgct gtatggcttc ttctttgcct
      721 cactgcagga cgatgccctg tgtccttatg tgtgtcacct gctgtatctg ctgaccaaaa
      781 aagaaaacgt caaacctttc cgtgtccgta aactgctgga cctgcaagcc aaa
//
