LOCUS       sequence-129104-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA protease chain with Rapamycin FKBP ectodomain and no linker 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: FKBP_LD 0_TEV
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             158..184
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(191..193,194..514)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     misc_feature    576..628
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgccatca tgtgccgagc catctctctt aggcgcttgc tgctgctgct
      121 gctgcagctg tcacaactcc tagctgtcac tcaagggtac ccctatgatg tgccagacta
      181 cgccggtacc atgggagtgc aggtggaaac catctcccca ggagacgggc gcaccttccc
      241 caagcgcggc cagacctgcg tggtgcacta caccgggatg cttgaagatg gaaagaaatt
      301 tgattcctcc cgggacagaa acaagccctt taagtttatg ctaggcaagc aggaggtgat
      361 ccgaggctgg gaagaagggg ttgcccagat gagtgtgggt cagagagcca aactgactat
      421 atctccagat tatgcctatg gtgccactgg gcacccaggc atcatcccac cacatgccac
      481 tctcgtcttc gatgtggagc ttctaaaact ggaaagtgct ggtggtagtg ctggtggtag
      541 tgctggtggt agtgctggtg gtagtgctgg tggtcctcga gctcaagctt cgaattctgc
      601 agtcgacggt accgcgggcc cgggatccac cggatctaga accggtaagc ttttttgggc
      661 actggtcgtg gttgctggag tcctgttttg ttatggcttg ctagtgacag tggctctttg
      721 tgttgagagc ttgtttaagg ggccgcgtga ttacaacccg atatcgagca ccatttgtca
      781 tttgacgaa
//
