LOCUS       sequence-129107-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-GFP(S65T)-His3MX6
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             383..397
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
ORIGIN
        1 gcccaaaaaa agcaaaaaca aactgttcag gagcgcaagg cgtttatctc ccgtatcact
       61 aatgaaacta aaattcaaat cgctatttcg ctgaatggtg gttatattca aataaaagat
      121 tcgattcttc ctgcaaagaa ggatgacgat gtagcttccc aagctactca gtcacaggtc
      181 atcgatattc acacaggtgt tggctttttg gatcatatga tccatgcgtt ggcaaaacac
      241 tctggttggt ctcttattgt tgaatgtatt ggtgacctgc acattgacga tcaccatact
      301 accgaagatt gcggtatcgc attagggcaa gcgttcaaag aagcaatggg tgctgtccgt
      361 ggtgtaaaaa gattcggtac tgggttcgca ccattggatg aggcgctatc acgtgccgta
      421 gtcgatttat ctagtagacc atttgctgta atcgaccttg gattgaagag agagatgatt
      481 ggtgatttat ccactgaaat gattccacac tttttggaaa gtttcgcgga ggcggccaga
      541 attactttgc atgttgattg tctgagaggt ttcaacgatc accacagaag tgagagtgcg
      601 ttcaaggctt tggctgttgc cataagagaa gctatttcta gcaatggcac caatgacgtt
      661 ccctcaacca aaggtgtttt gatgtgaagt actgacaata aaaagattct tgttttcaag
      721 aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc
      781 gtgatttata tttttttt
//
