LOCUS       sequence-129108-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Huang L, Hung L, Odell M, Yokota H, Kim R, Kim SH
  TITLE     Structure-based experimental confirmation of biochemical function to
            a methyltransferase, MJ0882, from hyperthermophile Methanococcus 
            jannaschii.
  JOURNAL   J Struct Funct Genomics. 2002  . 2(3):121-7.
  PUBMED    12836702
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Struct 
            Funct Genomics. 2002  . 2(3):121-7."
COMMENT     Sequence Label: pET21a.MJ0882
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..42)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             690..712
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    727..751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag
       61 cttgtcgacg gagctcgaat tcggatcctc ataatttctt actttttaag accctataac
      121 ctccttttat tgtaactgtc tcaacatttc caaagacatc tttcatatat ttagctaatg
      181 acttagcccc ttgctttgtt tgaataacta cccaaatctc gccattatct tttaaaagtt
      241 ctttaccttc ctcaataatt ctatgtaaaa cttcctttcc agctcttatt ggtggatttg
      301 ttataatctt attatacttt ctgtctttaa cattttcata taaatcgcta tgaactaccc
      361 taatatcata attatctaaa ttatttagtt ttatattctc tttggctaat tttattgccc
      421 tcctgtttat gtcagccatt gtagttgatt taacttcatc agctaaggca atgccaataa
      481 caccataacc acagcccaaa tccaagatgt cgtcatcttt atcaacaact acgttttcaa
      541 ctaaaatttt tgttccttta tcaacctttc cataagagaa aaccccacta tctgttttaa
      601 attttaattt ttttcctctt aaaatgtctt caacaatttt tacatctgat ttagttgttg
      661 gcttttcaga gaaatagtgc atatgtatat ctccttctta aagttaaaca aaattatttc
      721 tagaggggaa ttgttatccg ctcacaattc ccctata
//
