LOCUS       sequence-129109-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Lai L, Yokota H, Hung LW, Kim R, Kim SH
  TITLE     Crystal structure of archaeal RNase HII: a homologue of human major 
            RNase H.
  JOURNAL   Structure. 2000 Aug 15. 8(8):897-904.
  PUBMED    10997908
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Structure. 
            2000 Aug 15. 8(8):897-904."
COMMENT     Sequence Label: pET21a.MJ0135
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             774..791
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(844..862)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      858..905
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgata attattggta ttgatgaagc
       61 tggaagaggg cctgttttgg gtccaatggt tgtttgtgct tttgcaattg aaaaagagag
      121 agaagaggaa ttaaagaaat tgggagttaa agatagtaag gagctaacta aaaataaaag
      181 ggcttatcta aaaaagttac ttgaaaattt gggttatgta gaaaaacgta tcttagaagc
      241 agaggaaata aaccaattaa tgaactcaat aaacttaaat gatattgaaa ttaacgcctt
      301 ttccaaggtt gctaaaaatt tgatagaaaa gttgaatata agggatgatg aaattgaaat
      361 ttatattgat gcatgcagta caaacactaa aaaatttgag gatagtttta aagataagat
      421 agaggatata attaaagaaa gaaatttaaa cataaaaatt atagctgaac ataaggcaga
      481 tgctaagtat cctgttgttt cagctgcctc aataatagca aaggcagaac gggatgagat
      541 aatagactac tacaaaaaaa tttatggaga tattgggagc ggttatccat cagacccaaa
      601 aactataaag tttcttgaag attactttaa aaagcataaa aaacttcctg atattgcaag
      661 aactcactgg aaaacatgta aaaggatatt ggataaatca aaacagacaa agctaattat
      721 agagtgagga tccgaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc
      781 accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      841 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      901 ttttgctgaa aggaggaact atatccggat tgg
//
