LOCUS       sequence-129115-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa 
            scaffold, 6 aa linker domain, C-terminal TEV protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: FRB_SCF2_LD6_CTEV
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgccatca tgtgccgagc catctctctt aggcgcttgc tgctgctgct
      121 gctgcagctg tcacaactcc tagctgtcac tcaagggatg ctcgagatgt ggcatgaagg
      181 cctggaagag gcatctcgtt tgtactttgg ggaaaggaac gtgaaaggca tgtttgaggt
      241 gctggagccc ttgcatgcta tgatggaacg gggcccccag actctgaagg aaacatcctt
      301 taatcaggcc tatggtcgag atttaatgga ggcccaagag tggtgcagga agtacatgaa
      361 atcagggaat gtcaaggacc tcctccaagc ctgggacctc tattatcatg tgttccgacg
      421 aatctcaaag actagaaccg gtaagctttt ttgggcactg gtcgtggttg ctggagtcct
      481 gttttgttat ggcttgctag tgacagtggc tctttgtgtt atctgggtaa gatctggtat
      541 gtctagcatg gtgtcagaca ctagttgcac attcccttca tctgatggca tattctggaa
      601 gcattggatt caaaccaagg atgggcagtg tggcagtcca ttagtatcaa ctagagatgg
      661 gttcattgtt ggtatacact cagcatcgaa tttcaccaac acaaacaatt atttcacaag
      721 cgtgccgaaa aacttcatgg aattgttgac aaatcaggag gcgcagcagt gggttagtgg
      781 ttggcgatta aatgctgact cagtattgtg ggggggccat aaagttttca tggtgaaacc
      841 tgaagagcct tttcagccag ttaaggaagc gactcaactc atgaattagg cggccgccac
      901 cgcgg
//
