LOCUS       sequence-129116-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa 
            scaffold, 6 aa linker domain, C-terminal TEV protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: FRB_SCF2_LD6_CTEV
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggaaccttta atagaaattg gacagcaaga aagcgagctt agtgatactt gtgggccagg
       61 gcattagcca caccagccac cactttctga taggcagcct gcactggtgg ggtgaattgc
      121 tcgaggttta aacagctcca ccgcggtggc ggccgcctaa ttcatgagtt gagtcgcttc
      181 cttaactggc tgaaaaggct cttcaggttt caccatgaaa actttatggc ccccccacaa
      241 tactgagtca gcatttaatc gccaaccact aacccactgc tgcgcctcct gatttgtcaa
      301 caattccatg aagtttttcg gcacgcttgt gaaataattg tttgtgttgg tgaaattcga
      361 tgctgagtgt ataccaacaa tgaacccatc tctagttgat actaatggac tgccacactg
      421 cccatccttg gtttgaatcc aatgcttcca gaatatgcca tcagatgaag ggaatgtgca
      481 actagtgtct gacaccatgc tagacatacc agatcttacc cagataacac aaagagccac
      541 tgtcactagc aagccataac aaaacaggac tccagcaacc acgaccagtg cccaaaaaag
      601 cttaccggtt ctagtctttg agattcgtcg gaacacatga taatagaggt cccaggcttg
      661 gaggaggtcc ttgacattcc ctgatttcat gtacttcctg caccactctt gggcctccat
      721 taaatctcga ccataggcct gattaaagga tgtttccttc agagtctggg ggccccgttc
      781 catcatagca tgcaagggct ccagcacctc aaacatgcct ttcacgttcc tttccccaaa
      841 gtacaaacga gatgcctctt ccaggccttc atgccacatc tcgagcatcc cttgagtgac
      901 agctaggagt tgtgacagct gcagcagcag cagcagcaag cgcctaagag agatggctcg
      961 gcacatgatg gcg
//
