LOCUS       sequence-129127-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa 
            scaffold, 6 aa linker domain, N-terminal TEV protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: FKBP_SCF2_LD6_NTEV
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(171..173,174..494)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     misc_feature    556..608
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgcca tcatgtgccg agccatctct cttaggcgct
      121 tgctgctgct gctgctgcag ctgtcacaac tcctagctgt cactcaaggg atgggagtgc
      181 aggtggaaac catctcccca ggagacgggc gcaccttccc caagcgcggc cagacctgcg
      241 tggtgcacta caccgggatg cttgaagatg gaaagaaatt tgattcctcc cgggacagaa
      301 acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg
      361 ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat tatgcctatg
      421 gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc gatgtggagc
      481 ttctaaaact ggaaagtgct ggtggtagtg ctggtggtag tgctggtggt agtgctggtg
      541 gtagtgctgg tggtcctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc
      601 cgggatccac cggatctaga accggtaagc ttttttgggc actggtcgtg gttgctggag
      661 tcctgttttg ttatggcttg ctagtgacag tggctctttg tgttatctgg gtaagatctg
      721 gtgagagctt gtttaagggg ccgcgtgatt acaacccgat atcgagcacc atttgtcatt
      781 tgacgaatga atctgatggg cacacaacat cgttgtatgg tattggattt ggtcccttca
      841 tcattacaaa
//
