LOCUS       sequence-129129-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA target chain with Rapamycin FRB ectodomain, no linker domain, G
            cleavage sequence, and tTA-BFP fusion .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: FRB_LD 0_CS G_tTA-BFP
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             521..541
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(615..634)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgccatca tgtgccgagc catctctctt aggcgcttgc tgctgctgct
      121 gctgcagctg tcacaactcc tagctgtcac tcaagggatg ctcgagatgt ggcatgaagg
      181 cctggaagag gcatctcgtt tgtactttgg ggaaaggaac gtgaaaggca tgtttgaggt
      241 gctggagccc ttgcatgcta tgatggaacg gggcccccag actctgaagg aaacatcctt
      301 taatcaggcc tatggtcgag atttaatgga ggcccaagag tggtgcagga agtacatgaa
      361 atcagggaat gtcaaggacc tcctccaagc ctgggacctc tattatcatg tgttccgacg
      421 aatctcaaag actagaaccg gtaagctttt ttgggcactg gtcgtggttg ctggagtcct
      481 gttttgttat ggcttgctag tgacagtggc tctttgtgtt gaaaacctgt attttcaggg
      541 tatgtctaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      601 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      661 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      721 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      781 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      841 tttaggtaca c
//
