LOCUS       sequence-129130-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA target chain with Rapamycin FRB ectodomain, no linker domain, G
            cleavage sequence, and tTA-BFP fusion .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: FRB_LD 0_CS G_tTA-BFP
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(161..874,875..877,878..880))
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     814..835
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aaggaacctt taatagaaat tggacagcaa gaaagcgagc ttagtgatac ttgtgggcca
       61 gggcattagc cacaccagcc accactttct gataggcagc ctgcactggt ggggtgaatt
      121 gctcgaggtt taaacagctc caccgcggtg gcggccgcta ttacttgtac agctcgtcca
      181 tgccgagagt gatcccggcg gcggtgcgga actccagcag gaccatgtga tcgcgcttct
      241 cgttggggtc tttgctcagc acggactggg tgctcaggta gtggctgtcg ggcagcagca
      301 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
      361 cctccacgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgacggcca
      421 tgatatagat gttgtggctg ttgaagttgt actccagctt gtgccccagg atgttgccgt
      481 cctccttgaa gtcgacgccc ttcagctcga tgcggttcac tagggtgtcg ccctcgaact
      541 tcacctcggc gcgggtcttg taggtgccgt cgtccttgaa gaagatggtg cgctcctgga
      601 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
      661 gggcgaagca ctgcacgccg tggctcaggg tggtcacgag ggtgggccag ggcacgggca
      721 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gttggtggca tcgccctcgc
      781 cctcgcccct cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
      841 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat agagccacca ccggagcctc
      901 cgccggtacc
//
