LOCUS       sequence-129138-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations 
            with an N-terminal EGFP tag and C-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Sillibourne JE, Tack F, Vloemans N, Boeckx A, Thambirajah S, Bonnet 
            P, Ramaekers FC, Bornens M, Grand-Perret T
  TITLE     Autophosphorylation of polo-like kinase 4 and its role in centriole 
            duplication.
  JOURNAL   Mol Biol Cell. 2010 Feb 15;21(4):547-61. doi: 
            10.1091/mbc.E09-06-0505. Epub 2009 Dec 23.
  PUBMED    20032307
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-06-0505"; journalName: "Mol Biol Cell"; date: 
            "2010-02-15- 15"; volume: "21"; issue: "4"; pages: "547-61"
COMMENT     Sequence Label: pEGFP-C3-PLK4-S285A/T289A-3xFLAG
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     286..307
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
       61 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
      121 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
      181 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      241 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      301 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt actcagatct
      361 cgagctcaag cttggtacca tggcgacctg catcggggag aagatcgagg attttaaagt
      421 tggaaatctg cttggtaaag gatcatttgc tggtgtctac agagctgagt ccattcacac
      481 tggtttggaa gttgcaatca aaatgataga taagaaagcc atgtacaaag caggaatggt
      541 acagagagtc caaaatgagg tgaaaataca ttgccaattg aaacatcctt ctatcttgga
      601 gctttataac tattttgaag atagcaatta tgtgtatctg gtattagaaa tgtgccataa
      661 tggagaaatg aacaggtatc taaagaatag agtgaaaccc ttctcagaaa atgaagctcg
      721 acacttcatg caccagatca tcacagggat gttgtatctt cattctcatg gtatactaca
      781 ccgggacctc acactttcta acctcctact gactcgtaat atgaacatca agattgctga
      841 ttttggg
//
