LOCUS       sequence-129139-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations 
            with an N-terminal EGFP tag and C-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Sillibourne JE, Tack F, Vloemans N, Boeckx A, Thambirajah S, Bonnet 
            P, Ramaekers FC, Bornens M, Grand-Perret T
  TITLE     Autophosphorylation of polo-like kinase 4 and its role in centriole 
            duplication.
  JOURNAL   Mol Biol Cell. 2010 Feb 15;21(4):547-61. doi: 
            10.1091/mbc.E09-06-0505. Epub 2009 Dec 23.
  PUBMED    20032307
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-06-0505"; journalName: "Mol Biol Cell"; date: 
            "2010-02-15- 15"; volume: "21"; issue: "4"; pages: "547-61"
COMMENT     Sequence Label: pEGFP-C3-PLK4-S285A/T289A-3xFLAG
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(133..198)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca gttatctaga
      121 tccggtggat cctttgtcat cgtcatcctt gtaatcgata tcatgatctt tataatcacc
      181 gtcatggtct ttgtagtcca tcgtcgactg cagaattcga agcttatgaa aattaggagt
      241 cggattagaa aacatcaaaa ggatggaaga cagacactgt aatttctgtt tgatgtagtc
      301 tggtaatttt tcattttctc catacctagt tgtttgacca tttggtgagg tataactgat
      361 agaagacact cctgcctgca caaccaactg ggacccatca ttaaactgaa cccacacagc
      421 tccactagtt aactgtgtag cccaaccaac atttttcaca aaaacagatt tcaaaagttg
      481 tgctgattta ggaagacaat cttttagact attagaagag atgtctgttc cagaagctgt
      541 agttgtaaga ccaagtcctt catttgtaac catagaggga ttaaggattg gtgcctgtgt
      601 tggagaagca gcactatgca tgaccattct gttgaaagat gctctatctc tcgttgggta
      661 atttgagtcc acagaaggag gaggtgataa ggccttaggt gaactagtac taccaggttt
      721 tcttcctatg attattggga aaaagggagc actcctagtt ttcctttcct cttctgaaat
      781 tatggattcc agtgctaaac aaatacgatg accctcatta gcatggtcca tatacatttt
      841 tatctcctct ttcaagctat taacttcact ttcacttttt aaagtgtaag acttccctgt
      901 cttttcaatc acctgaatga aatcttctgt tttgtgtatt tttacccc
//
