LOCUS       sequence-129140-         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes kinase dead PLK4 isoform 1 carrying K41M and 
            S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 
            3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Sillibourne JE, Tack F, Vloemans N, Boeckx A, Thambirajah S, Bonnet 
            P, Ramaekers FC, Bornens M, Grand-Perret T
  TITLE     Autophosphorylation of polo-like kinase 4 and its role in centriole 
            duplication.
  JOURNAL   Mol Biol Cell. 2010 Feb 15;21(4):547-61. doi: 
            10.1091/mbc.E09-06-0505. Epub 2009 Dec 23.
  PUBMED    20032307
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-06-0505"; journalName: "Mol Biol Cell"; date: 
            "2010-02-15- 15"; volume: "21"; issue: "4"; pages: "547-61"
COMMENT     Sequence Label: pEGFP-C3-PLK4-K41M-S285A-T289A-3xFLAG
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttgatgctgg gcatgccgca atttctactg caattacagc ttcttccagt accagtataa
       61 gtggtagttt atttgacaaa agaagacttt tgattggtca gccactccca aataaaatga
      121 ctgtatttcc aaagaataaa agttcaactg atttttcttc ttcaggagat ggaaacagtt
      181 tttatactca gtggggaaat caagaaacca gtaatagtgg aaggggaaga gtaattcaag
      241 atgcagaaga aaggccacat tctcgatacc ttcgtagagc ttattcctct gatagatctg
      301 gcacttctaa tagtcagtct caagcaaaaa catatacaat ggaacgatgt cactcagcag
      361 aaatgctttc agtgtccaaa agatcaggag gaggtgaaaa tgaagagagg tactcaccca
      421 cagacaacaa tgccaacatt tttaacttct ttaaagaaaa gacatccagt agttctggat
      481 cttttgaaag acctgataac aatcaagcac tctccaatca tctttgtcca ggaaaaactc
      541 cttttccatt tgcagacccg acacctcaga ctgaaaccgt acaacagtgg tttgggaatc
      601 tgcaaataaa tgctcattta agaaaaacta ctgaatatga cagcatcagc ccaaacc
//
