LOCUS       sequence-129143-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for bacterial expression; His,GST tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  TITLE     Gateway Destination vectors for E. coli expression
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDest-565
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(24..30,31..48,49..54))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(79..203)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(210..227)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(254..276)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg taaagcctgg
       61 ggtgcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
      121 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
      181 tcagcttttt tgtacaaact tgtgatatca gatccacgcg gaaccagatc cgattttgga
      241 ggatggtcgc caccaccaaa cgtggcttgc cagccctgca aaggccatgc tatatacttg
      301 ctggatttca agtacttatc aatttgtggg atagcttcaa tacgtttttt aaaacaaact
      361 aattttggga acgcatccag gcacattggg tccatgtata aaacaacatc aagagcgtca
      421 tacaacatga agtcaggatg ggttacatga tcaccattta aatatgtttt atgacataaa
      481 cgatcttcga acattttcag catttcaggt agcttgctaa gaaaatcaac tttgagagtt
      541 tcaaagtctt tactatatgc aattctcgaa acaccgtatc taatatccaa aaccgctcct
      601 tcaagcattg aaatctctgc acgctctttt ggacaaccac ccaacatgtt gtgcttgtca
      661 gctatataac gtatgatggc catagactgt gttaatttaa catcaccatc aatataataa
      721 ggaagat
//
