LOCUS       sequence-129145-         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA to human HOXB13, puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Pomerantz MM, Li F, Takeda DY, Lenci R, Chonkar A, Chabot M, Cejas 
            P, Vazquez F, Cook J, Shivdasani RA, Bowden M, Lis R, Hahn WC, 
            Kantoff PW, Brown M, Loda M, Long HW, Freedman ML
  TITLE     The androgen receptor cistrome is extensively reprogrammed in human 
            prostate tumorigenesis.
  JOURNAL   Nat Genet. 2015 Nov;47(11):1346-51. doi: 10.1038/ng.3419. Epub 2015 
            Oct 12.
  PUBMED    26457646
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.3419"; 
            journalName: "Nat Genet"; date: "2015-11"; volume: "47"; issue: 
            "11"; pages: "1346-51"
COMMENT     Sequence Label: pLKO_HOXB13_#1
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             55..288
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(499..519)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ttcatttctt tgcaagttat aaatactgaa taataagatg acatgaacta ctactgctag
       61 agattttcca cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca
      121 gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt
      181 tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac
      241 aagcaaaaag cagatcttgt cttcgttggg agtgaattag cccttccagt cccccctttt
      301 cttttaaaaa gtggctaaga tctacagctg ccttgtaagt cattggtctt aaaggtaccg
      361 atgcatgggg tcgtgcgctc ctttcggtcg ggcgctgcgg gtcgtggggc gggcgtcagg
      421 caccgggctt gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg
      481 tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga
      541 aggcgggcac cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca
      601 gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct
      661 tgggccggtg cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaa
//
