LOCUS       sequence-129152-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA to human FOXA1, puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Pomerantz MM, Li F, Takeda DY, Lenci R, Chonkar A, Chabot M, Cejas 
            P, Vazquez F, Cook J, Shivdasani RA, Bowden M, Lis R, Hahn WC, 
            Kantoff PW, Brown M, Loda M, Long HW, Freedman ML
  TITLE     The androgen receptor cistrome is extensively reprogrammed in human 
            prostate tumorigenesis.
  JOURNAL   Nat Genet. 2015 Nov;47(11):1346-51. doi: 10.1038/ng.3419. Epub 2015 
            Oct 12.
  PUBMED    26457646
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.3419"; 
            journalName: "Nat Genet"; date: "2015-11"; volume: "47"; issue: 
            "11"; pages: "1346-51"
COMMENT     Sequence Label: pLKO_FOXA1_#2
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             41..274
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(485..505)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 agttataaat actgaataat aagatgacat gaactactac tgctagagat tttccacact
       61 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact
      121 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca
      181 gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaagc aaaaagcaga
      241 tcttgtcttc gttgggagtg aattagccct tccagtcccc ccttttcttt taaaaagtgg
      301 ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag gtaccgatgc atggggtcgt
      361 gcgctccttt cggtcgggcg ctgcgggtcg tggggcgggc gtcaggcacc gggcttgcgg
      421 gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt cggcggtgac ggtgaagccg
      481 agccgctcgt agaaggggag gttgcggggc gcggaggtct ccaggaaggc gggcaccccg
      541 gcgcgctcgg ccgcctccac tccggggagc acgacggcgc tgcccagacc cttgccctgg
      601 tggtcgggcg agacgccgac ggtggccagg aaccacgcgg gctccttggg ccggtgcggc
      661 gccaggaggc cttccatctg ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg
      721 cgcgggccga tctcggcgaa caccgccccc gcttcgacg
//
