LOCUS       sequence-129161-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase 
            in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain 
            compatibility with the F2/R1 primer pairs commonly used for epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Zentner GE, Kasinathan S, Xin B, Rohs R, Henikoff S
  TITLE     ChEC-seq kinetics discriminates transcription factor binding sites 
            by DNA sequence and shape in vivo.
  JOURNAL   Nat Commun. 2015 Oct 22;6:8733. doi: 10.1038/ncomms9733.
  PUBMED    26490019
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-22"; volume: "6:8733. doi"; pages: " 
            10.1038/ncomms9733"
COMMENT     Sequence Label: pGZ108 (pFA6a-3FLAG-MNase-kanMX6)
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(76..273)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 cactagtgga tctgatatca tcgatgaatt cgagctcgtt taaactggat ggcggcgtta
       61 gtatcgaatc gacagcagta tagcgaccag cattcacata cgattgacgc atgatattac
      121 tttctgcgca cttaacttcg catctgggca gatgatgtcg aggcgaaaaa aaatataaat
      181 cacgctaaca tttgattaaa atagaacaac tacaatataa aaaaactata caaatgacaa
      241 gttcttgaaa acaagaatct ttttattgtc agtactgatt agaaaaactc atcgagcatc
      301 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
      361 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
      421 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
      481 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
      541 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
      601 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
      661 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
      721 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
      781 gttttgccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
      841 ttgatggtcg ga
//
