LOCUS       sequence-129163-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in 
            pFA6a-3HA- HIS3MX6 replacing the 3xHA tag. These vectors retain 
            compatibility with the F2/R1 primer pairs commonly used for epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Zentner GE, Kasinathan S, Xin B, Rohs R, Henikoff S
  TITLE     ChEC-seq kinetics discriminates transcription factor binding sites 
            by DNA sequence and shape in vivo.
  JOURNAL   Nat Commun. 2015 Oct 22;6:8733. doi: 10.1038/ncomms9733.
  PUBMED    26490019
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-22"; volume: "6:8733. doi"; pages: " 
            10.1038/ncomms9733"
COMMENT     Sequence Label: pGZ109 (pFA6a-3FLAG-MNase-HIS3MX6)
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..100
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             107..553
                     /codon_start=1
                     /product="micrococcal nuclease from Staphylococcus aureus"
                     /label=MNase
                     /translation="ATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETK
                     HPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALV
                     RQGLAKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEDNADSGQ"
     terminator      581..768
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(636..660)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 cgctgcaggt cgacggatcc ccgggttaat taaagattac aaggatcacg atggcgatta
       61 caaggatcac gatatcgatt acaaggatga tgatgataag gcggccgcca ccagtaccaa
      121 gaagctgcac aaggagcccg ccaccctgat caaggccata gatggcgata ccgtgaagct
      181 gatgtataag ggccagccca tgaccttccg cctgctgctg gtggataccc ccgagaccaa
      241 gcacccgaaa aagggcgtgg aaaagtacgg acccgaggcc agcgccttca caaaaaagat
      301 ggtggagaac gccaagaaaa tcgaggtgga gttcgataaa ggccaacgca ccgataagta
      361 tggacgcggt ctggcctaca tctacgccga cggcaaaatg gtgaacgagg ccctggtgcg
      421 gcagggactg gccaaggtgg cgtacgtgta caagcccaac aacacccacg agcagcacct
      481 gcgcaagagc gaggctcagg caaagaagga gaaactgaac atctggagtg aggataacgc
      541 cgatagcggc cagtagtaga ggcgcgccac ttctaaataa gcgaatttct tatgatttat
      601 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
      661 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct
      721 ttctcaggta tagtatgagg tcgctcttat tgaccacacc tctaccggca gatccgctag
      781 ggataacagg gtaatataga tctgtttagc ttgcctcgtc cccgccgggt cacccggcca
      841 gcgacatgga ggcccagaat accctccttg acagtcttga cgtgcgcagc tc
//
