LOCUS       sequence-129165-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in 
            pFA6a-3HA- HIS3MX6 replacing the 3xHA tag. These vectors retain 
            compatibility with the F2/R1 primer pairs commonly used for epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Zentner GE, Kasinathan S, Xin B, Rohs R, Henikoff S
  TITLE     ChEC-seq kinetics discriminates transcription factor binding sites 
            by DNA sequence and shape in vivo.
  JOURNAL   Nat Commun. 2015 Oct 22;6:8733. doi: 10.1038/ncomms9733.
  PUBMED    26490019
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-22"; volume: "6:8733. doi"; pages: " 
            10.1038/ncomms9733"
COMMENT     Sequence Label: pGZ109 (pFA6a-3FLAG-MNase-HIS3MX6)
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(73..270)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(564..578)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
ORIGIN
        1 tagtggatct gatatcatcg atgaattcga gctcgtttaa actggatggc ggcgttagta
       61 tcgaatcgac agcagtatag cgaccagcat tcacatacga ttgacgcatg atattacttt
      121 ctgcgcactt aacttcgcat ctgggcagat gatgtcgagg cgaaaaaaaa tataaatcac
      181 gctaacattt gattaaaata gaacaactac aatataaaaa aactatacaa atgacaagtt
      241 cttgaaaaca agaatctttt tattgtcagt acttcacatc aaaacacctt tggttgaggg
      301 aacgtcattg gtgccattgc tagaaatagc ttctcttatg gcaacagcca aagccttgaa
      361 cgcactctca cttctgtggt gatcgttgaa acctctcaga caatcaacat gcaaagtaat
      421 tctggccgcc tccgcgaaac tttccaaaaa gtgtggaatc atttcagtgg ataaatcacc
      481 aatcatctct ctcttcaatc caaggtcgat tacagcaaat ggtctactag ataaatcgac
      541 tacggcacgt gatagcgcct catccaatgg tgcgaaccca gtaccgaatc tttttacacc
      601 acggacagca cccattgctt ctttgaacgc ttgccctaat gcgataccgc aatcttcggt
      661 agtatggtga tcgtcaatgt gcaggtcacc aatacattca acaataagag accaaccaga
      721 gtgttttgcc aacgcatgga tcatatgatc caaaaagcca acacctgtgt gaatatcgat
      781 gacctgtgac tgagtagctt gggaagctac atcgtcatcc ttctttgcag gaagaatcga
      841 atcttttatt tgaatataac caccattcag cgaaatagcg atttgaat
//
