LOCUS       sequence-129166-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in 
            pFA6a-3HA-Trp1 replacing the 3xHA tag. These vectors retain 
            compatibility with the F2/R1 primer pairs commonly used for epitope 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Zentner GE, Kasinathan S, Xin B, Rohs R, Henikoff S
  TITLE     ChEC-seq kinetics discriminates transcription factor binding sites 
            by DNA sequence and shape in vivo.
  JOURNAL   Nat Commun. 2015 Oct 22;6:8733. doi: 10.1038/ncomms9733.
  PUBMED    26490019
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-22"; volume: "6:8733. doi"; pages: " 
            10.1038/ncomms9733"
COMMENT     Sequence Label: pGZ110 (pFA6a-3FLAG-MNase-TRP1)
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..101
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             108..554
                     /codon_start=1
                     /product="micrococcal nuclease from Staphylococcus aureus"
                     /label=MNase
                     /translation="ATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETK
                     HPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALV
                     RQGLAKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEDNADSGQ"
     terminator      582..769
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(637..661)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 acgctgcagg tcgacggatc cccgggttaa ttaaagatta caaggatcac gatggcgatt
       61 acaaggatca cgatatcgat tacaaggatg atgatgataa ggcggccgcc accagtacca
      121 agaagctgca caaggagccc gccaccctga tcaaggccat agatggcgat accgtgaagc
      181 tgatgtataa gggccagccc atgaccttcc gcctgctgct ggtggatacc cccgagacca
      241 agcacccgaa aaagggcgtg gaaaagtacg gacccgaggc cagcgccttc acaaaaaaga
      301 tggtggagaa cgccaagaaa atcgaggtgg agttcgataa aggccaacgc accgataagt
      361 atggacgcgg tctggcctac atctacgccg acggcaaaat ggtgaacgag gccctggtgc
      421 ggcagggact ggccaaggtg gcgtacgtgt acaagcccaa caacacccac gagcagcacc
      481 tgcgcaagag cgaggctcag gcaaagaagg agaaactgaa catctggagt gaggataacg
      541 ccgatagcgg ccagtagtag aggcgcgcca cttctaaata agcgaatttc ttatgattta
      601 tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact
      661 cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc
      721 tttctcaggt atagtatgag gtcgctctta ttgaccacac ctctaccggc agatccgcta
      781 gggataacag ggtaatatag atctgtacaa tcttgatccg gagctt
//
