LOCUS       sequence-129167-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in 
            pFA6a-3HA-Trp1 replacing the 3xHA tag. These vectors retain 
            compatibility with the F2/R1 primer pairs commonly used for epitope 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Zentner GE, Kasinathan S, Xin B, Rohs R, Henikoff S
  TITLE     ChEC-seq kinetics discriminates transcription factor binding sites 
            by DNA sequence and shape in vivo.
  JOURNAL   Nat Commun. 2015 Oct 22;6:8733. doi: 10.1038/ncomms9733.
  PUBMED    26490019
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-22"; volume: "6:8733. doi"; pages: " 
            10.1038/ncomms9733"
COMMENT     Sequence Label: pGZ110 (pFA6a-3FLAG-MNase-TRP1)
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             41..49
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(129..803)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 tagtggatct gatatcatcg atgaattcga gctcgtttaa acctccttac gcatctgtgc
       61 ggtatttcac accgcataga tcggcaagtg cacaaacaat acttaaataa atactactca
      121 gtaataacct atttcttagc atttttgacg aaatttgcta ttttgttaga gtcttttaca
      181 ccatttgtct ccacacctcc gcttacatca acaccaataa cgccatttaa tctaagcgca
      241 tcaccaacat tttctggcgt cagtccacca gctaacataa aatgtaagct ttcggggctc
      301 tcttgccttc caacccagtc agaaatcgag ttccaatcca aaagttcacc tgtcccacct
      361 gcttctgaat caaacaaggg aataaacgaa tgaggtttct gtgaagctgc actgagtagt
      421 atgttgcagt cttttggaaa tacgagtctt ttaataactg gcaaaccgag gaactcttgg
      481 tattcttgcc acgactcatc tccatgcagt tggacgatat caatgccgta atcattgacc
      541 agagccaaaa catcctcctt aggttgatta cgaaacacgc caaccaagta tttcggagtg
      601 cctgaactat ttttatatgc ttttacaaga cttgaaattt tccttgcaat aaccgggtca
      661 attgttctct ttctattggg cacacatata atacccagca agtcagcatc ggaatctaga
      721 gcacattctg cggcctctgt gctctgcaag ccgcaaactt tcaccaatgg accagaacta
      781 cctgtgaaat taataacaga catactccaa gctgcctttg tgtgcttaat cacgtatact
      841 cacgtgctca atagtcacca atgccc
//
