LOCUS       sequence-129169-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase 
            in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain 
            compatibility with the F2/R1 primer pairs commonly used for epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Zentner GE, Kasinathan S, Xin B, Rohs R, Henikoff S
  TITLE     ChEC-seq kinetics discriminates transcription factor binding sites 
            by DNA sequence and shape in vivo.
  JOURNAL   Nat Commun. 2015 Oct 22;6:8733. doi: 10.1038/ncomms9733.
  PUBMED    26490019
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-22"; volume: "6:8733. doi"; pages: " 
            10.1038/ncomms9733"
COMMENT     Sequence Label: pGZ173 (pFA6a-SL-MNase-kanMX6)
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      508..695
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(563..587)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 tacgctgcag gtcgacggat ccccgggtta atcgccacca gtaccaagaa gctgcacaag
       61 gagcccgcca ccctgatcaa ggccatagat ggcgataccg tgaagctgat gtataagggc
      121 cagcccatga ccttccgcct gctgctggtg gatacccccg agaccaagca cccgaaaaag
      181 ggcgtggaaa agtacggacc cgaggccagc gcctccacaa aaaagatggt ggagaacgcc
      241 aagaaaatcg aggtggagtt cgataaaggc caacgcaccg ataagtatgg acgcggtctg
      301 gcctacatct acgccgacgg caaaatggtg aacgaggccc tggtgcggca gggactggcc
      361 aaggtggcgt acgtgtacaa gcccaacaac acccacgagc agcacctgcg caagagcgag
      421 gctcaggcaa agaaggagaa actgaacatc tggagtgagg ataacgccga tagcggccag
      481 tagtagaggc gcgccacttc taaataagcg aatttcttat gatttatgat ttttattatt
      541 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa
      601 cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag
      661 tatgaggtcg ctcttattga ccacacctct accggcagat ccgctaggga taacagggta
      721 atatagatct gtttagcttg cctcgtcccc gccgggtcac ccggccagcg acatggaggc
      781 ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg
      841 cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg
      901 ccgcacggcg cgaagca
//
