LOCUS       sequence-129172-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase 
            in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain 
            compatibility with the F2/R1 primer pairs commonly used for epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Zentner GE, Kasinathan S, Xin B, Rohs R, Henikoff S
  TITLE     ChEC-seq kinetics discriminates transcription factor binding sites 
            by DNA sequence and shape in vivo.
  JOURNAL   Nat Commun. 2015 Oct 22;6:8733. doi: 10.1038/ncomms9733.
  PUBMED    26490019
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-22"; volume: "6:8733. doi"; pages: " 
            10.1038/ncomms9733"
COMMENT     Sequence Label: pGZ173 (pFA6a-SL-MNase-kanMX6)
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(74..271)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ctagtggatc tgatatcatc gatgaattcg agctcgttta aactggatgg cggcgttagt
       61 atcgaatcga cagcagtata gcgaccagca ttcacatacg attgacgcat gatattactt
      121 tctgcgcact taacttcgca tctgggcaga tgatgtcgag gcgaaaaaaa atataaatca
      181 cgctaacatt tgattaaaat agaacaacta caatataaaa aaactataca aatgacaagt
      241 tcttgaaaac aagaatcttt ttattgtcag tactgattag aaaaactcat cgagcatcaa
      301 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
      361 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
      421 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
      481 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
      541 cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
      601 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
      661 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
      721 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
      781 tttgccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
      841 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
      901 atcattggca acgctacct
//
