LOCUS       sequence-129183-         381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes human full length SENP3. Suitable for in vitro 
            transcription/translation (T7)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Mendes AV, Grou CP, Azevedo JE, Pinto MP
  TITLE     Evaluation of the activity and substrate specificity of the human 
            SENP family of SUMO proteases.
  JOURNAL   Biochim Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 
            10.1016/j.bbamcr.2015.10.020.
  PUBMED    26522917
REFERENCE   2  (bases 1 to 381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 
            10.1016/j.bbamcr.2015.10.020."
COMMENT     Sequence Label: pET28a-HsSENP3
FEATURES             Location/Qualifiers
     source          1..381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..94
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca
       61 tcatcacagc agcggcctgg tgccgcgcgg cagccatatg aaagagacta tacaagggac
      121 cgggtcctgg gggcctgagc ctcctggacc cggcataccc ccagcttact caagtcccag
      181 gcgggagcgt cttcgttggc ccccacctcc caaaccccga ctcaagtcag gtggagggtt
      241 tgggccagat cctgggtcag ggaccacagt gccagccaga cgcctccctg tcccccgacc
      301 ctcttttgat gcctcagcaa gtgaagagga ggaagaagag gaggaggagg aggatgaaga
      361 tgaagaggag gaagtggcac c
//
