LOCUS       sequence-129202-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of Tousled-like kinase 1 (TLK1) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pIC214
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..68
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             81..125
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 agtccggagg ggatcggtgg agctccaccg gtggcggccg ctctagagag aatctttatt
       61 ttcagggcgc cgccaaattc aaagaaaccg ctgctgctaa attcgaacgc cagcacatgg
      121 acagcggagg tggaggtact agtctcgaga tgagtgtcca aagtagcagt ggaagtttgg
      181 aggggccgcc atcttggtcc cagctctcca cgtctccaac cccgggctcg gcggcggcgg
      241 ccaggtccct gctgaatcac acgccgccat ccgggaggcc cagggaaggt gcaatggatg
      301 agcttcatag tctggatcca agaaggcaag agttattgga agctagattt actggagttg
      361 caagtgggag cactggaagt acgggcagtt gcagtgttgg agctaaagcc tcaacaaata
      421 acgaaagctc taatcacagt tttggaagct tgggatcttt aagtgacaaa gaatcagaga
      481 caccggagaa gaaacaatcg gaatcatcca ggggaagaaa gagaaaagca gaaaaccaga
      541 atgaaagtag tcagggaaaa agtattgggg gacgtggcca caaaattagc gactattttg
      601 aataccaggg tggaaatggc tcaagtccag taagaggcat acctcctgca atccgttctc
      661 ctcaaaattc acattcacat tccactcctt cctcatctgt tcgaccgaat agcccttctc
      721 ctactgcatt agcatttgg
//
