LOCUS       sequence-129213-        7918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pUC19-TSV (linearized in VP2) contains a genome of the 
            Trichodysplasia spinulosa-associated polyomaviruse (GenBank 
            NC_014361) linearized and cloned in the VP2-coding region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   van der Meijden E, Janssens RW, Lauber C, Bouwes Bavinck JN, 
            Gorbalenya AE, Feltkamp MC
  TITLE     Discovery of a new human polyomavirus associated with 
            trichodysplasia spinulosa in an immunocompromized patient.
  JOURNAL   PLoS Pathog. 2010 Jul 29;6(7):e1001024. doi: 
            10.1371/journal.ppat.1001024.
  PUBMED    20686659
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2010-07-29"; volume: "6(7):e1001024. doi"; pages: "
            10.1371/journal.ppat.1001024"
COMMENT     Sequence Label: pUC19-TSV (linearized in VP2)
FEATURES             Location/Qualifiers
     source          1..7918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(51..67)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(51..67)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(64..86)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(99..105,106..123,124..129))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    144..165
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(282..299)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(453..1041)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(533..552)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1212..2003,2004..2072))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1835..1854
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2073..2177)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2245..2263
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2301..2323)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2423..2442
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2636..2658
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2650..2667
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2651..2667
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg
       61 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      121 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      181 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      241 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      301 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      361 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      421 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      481 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      541 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      601 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
      661 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
      721 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
      781 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
      841 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
      901 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
      961 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1021 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1081 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1141 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1201 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1261 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1321 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     1381 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1441 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1501 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     1561 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     1621 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     1681 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1741 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1801 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     1861 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     1921 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1981 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     2041 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     2101 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2161 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     2221 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     2281 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     2341 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     2401 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     2461 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     2521 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     2581 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     2641 tcacgacgtt gtaaaacgac ggccagtgaa ttcgagctcg gtacccatca acttgaggct
     2701 gccacagatg aatctgctga ggtaattgaa acctactcag ctccaggagg agcacatcaa
     2761 agagtctgcc ctgactggat gttgcctctg gtactgggat tgtatggtga tataaccccc
     2821 acttttggtt actatttgag agaagaggaa agagaagatg gcccccaaaa gaaaaggaga
     2881 aggatgcgct agaaagtgcc ccacaaaaac atgtcctaca cccaagcctg ttccaaaact
     2941 tattatgaag ggcaatatag aggttctaaa ccttgttaca ggccctgaca gtataacaac
     3001 aattgaactc tatcttaaca ccaggatggg acaaaatgat gagtctaagg acaactatgg
     3061 ttacagtgaa aaagtaactg ttgctaacag cagtgaccag gacaagccta cttctggaga
     3121 gataccaacc tacagtacag ctagaattaa tctacccatg ttgaatgagg acctaacctg
     3181 taatactcta actatgtggg aagctgtctc tgtaaaaact gaagtggttg gggttagctc
     3241 tctggtaaat gttcatatgg ctactaaaag aatgtatgat gacaaaggta ttggtttccc
     3301 agttgaggga atgaatttcc atatgtttgc agtgggtggg gaacccctag aactccagtt
     3361 tctaactgga aactacagaa ctgattacag tgctaatgac aaattggttg ttcccccaat
     3421 aaaacaccag agcacacaag gcctcaatcc tcactacaaa caaaagctaa caaaagatgg
     3481 ggcctttcct gtagagtgct ggtgcccaga cccttcaaaa aatgaaaata caagatatta
     3541 tggcagctat actggtggtc agagtacacc tccagttttg caatttacaa acactgtgac
     3601 tactgtgcta ctagatgaga atggtgttgg cccactttgt aaaggtgatg gcctgtatgt
     3661 gtcatgctgt gacatagtag gctttctggt tgggaaagat ggagacatgc aatacagagg
     3721 cctacctagg tactttaata ttctattgag aaagagaact gtaagaaatc cttacccagt
     3781 ttcttccttg ttgaataatc ttttcactgg tcttatgcca gctgttcagg gccagcctat
     3841 ggacaatgga ctaagtacac aggttgaaga agtcagagtt tatgatggaa ctgaaggcct
     3901 tcctggagat ccagacatgg tcagatatat tgacaagttt ggacaagaca aaacccgccc
     3961 ccccttcccc gcccggcttt attgagtcac aaacaaagta tttgctaaaa agctttatta
     4021 aatggcttgc tttccatgct gcaggttttg ctgttatata aaactaataa aggtttattg
     4081 tgtttggaaa ccagaatcat ttgtttcaga gaattcttct tcctcctcat cctccacctc
     4141 aattacaaca gaatcaagag gatctttacc ctcctccaca tttaatagca aggtagcaaa
     4201 attagtatga cccatgtatt tacatagaat atctttccaa tacttaacat cctcctttat
     4261 tgactctgaa aacatggtaa ttggacagta ccaaaccaac aacaaagcaa tagtgactcc
     4321 atcctgtagt attctttctg ttaataagta gtcactaacc tgaaggcaag atctaagatt
     4381 atgctttgga gtaaaattca aaacataagc aaaccttgta aaaatggttt caggcaagag
     4441 gtactcattc atagtcacaa gacagggtgg gaagatctgg cttcttttgt tcacatgctt
     4501 cctttctaaa tttactttaa cactgccatc cagatggtct cttaaattat ctaaattaga
     4561 tactccctgt ccaggctgca gctttttatt aagtgctatt tgacccttga catcttcaaa
     4621 aataactaca aactggtcta ttgcacatcc aagttcaaag ggcaatttat ctgcaggaca
     4681 attgacattt aatgtttttc ctcccaggaa attcatcaga gcagctgcaa aagtagtttt
     4741 cccactattg attggtcctc taaaaaggca atttcttttt ttagggacat tttctacaat
     4801 taactttaag attttggtga ctacttcatc tatattttca aataagcaag aataccaagc
     4861 aactcccgcc atatactgaa gaattttaat ttgaccaaac tcatcagtaa gtttctcaaa
     4921 cattaattta aatctttcct ccagtaattc ttttctagta ctttcaatta actttaatct
     4981 ctgttttgcc atcaccacat ctgctgcttg ttggcaggca gttttttgag ttttgcactc
     5041 cttaaacagc tttgcatttt tatgatgtaa gctatggtaa ttatagtgag cttttagagc
     5101 ttttttagtg catttagagc atatgcttgg ctcttctgca aagtctaagt aatgagccat
     5161 aattaacagg ggatcatcta gcctgttggt aactgcaaaa tcagtgagaa ggttccaatt
     5221 aaccacaggc ttcccatttt cttcattaaa ctcatattca aatattcctg gcttgctttc
     5281 ctccagtagc ttaaatggtt cactgcacat acatctgtaa cattctactt gtttaattac
     5341 tgctttgcat aacaagaaac taacagtgca gtgtgttaca cataaattct taattgctga
     5401 aactctatgc ttgccaggag tcattaggaa taccatgctc ccttcctgaa aagagtgcct
     5461 acttttaaat tcaggattaa atttatcaag ctttttatac aaatattcag ctttttcatt
     5521 agttgtatat atcagaaaag atgataaagt tttattacta tagacagcat tagaaagaaa
     5581 aggccgcaac atatcaggaa aatcattagg gacagagtca tacttagatt tttttggctt
     5641 tgggggggtg gcagagaagg agctctgggt gcagccgggg gagtcctctg gtggggcagc
     5701 tcttcttctt cttgaattct cctcgctaaa ttgagtagag ggtcccggag tggggcttgg
     5761 agatctagat tcatcagagg gaatggtgga ttcatggcag aagagatctg ggtcctgcat
     5821 ggtgtcaaac aagttgtccc attcttgatt gaagctttcc caccagctgg cccaggaggg
     5881 gtgcccatag ggaggtatat ctcttccatc ctgggttgga acatcatgct aaaaggaaaa
     5941 actaaattaa tataggtaca taacacctgc agtttcccca ccttttactt accccagtta
     6001 aagccttgat taactctcag atagagattc accaggtgaa agtcaacttc tcctactatg
     6061 caactccaag cccaaaggga atccaagcaa tataagtctt ctccaaacca tgcacagtag
     6121 catttgtaac aaaagcattc tccccaaact acacacttct tctggtgcat ttgcttataa
     6181 acatgatgtt gaaggcctat tttgcaggtt aagcatttac actcttttaa agctttttct
     6241 gcacactgag gccaatgttt aattacattc tcatcatatt tttttttccc aaaatattcc
     6301 ttcaaagata ttaaatttgc ctcccaatac catgagccta cctggctact gaagctagtt
     6361 ggaaactctt gtcttgcatt ataaattcct tcttgcagtt tttgccataa ttgatttaat
     6421 cttgacattt tttctggatc tcctccttta tctggatggt atttcaagct cattttttta
     6481 tgattaattt tcattagtgc aaaattccca tagcaatgcc ttgggatttg caataaatcc
     6541 ataagttcaa gactttcttc tctacttaaa aacttatcca ttttgctgaa tgcaccagaa
     6601 gacaggtaag gggagaaatg aagaaatgaa gatgggagct ttttggagcc cttttaaagc
     6661 ctctgtgtgc ctcaattaat tttcatcaga tttggcttac ataaaaggga ggaagtgcca
     6721 agcctcaaga ggcctccgga ggcctctccc tctatgtctg tgtcaagagg gggcaggaag
     6781 cctcagcctc ctggtaatac tgagaaaaaa caagatttcc atagtaaccc cctattagga
     6841 atttgaggaa ctatacttag agtgttgaca taaataggtc attgtttgct taagaccgtt
     6901 atcctgagga agctcgtgcg ccacctatca tttgttatgg aaaatgctgt gtcagttagt
     6961 ctcaagggga tgtttgtcat actgccacaa acacaggaag gcagccaaga gacaagagga
     7021 agccagccaa atcccgtgat tgtttttgta tattcttaag atagcacttg tctaactgct
     7081 ctcatcctgt ccgtccggtc tccataacaa cacatcctcc cgctctgtgt tctcacgcgg
     7141 caaattcaaa ttctaatggt aagtttttct tatttttcag gtaaaatggg aggagtaatc
     7201 agcttattct ttgatattat tgaaatatca acagaattaa gtgctgctac aggatttgct
     7261 gtggatgctg tgttagcagg agaagcagca gctgctgttg aagttgaagt aatgggccta
     7321 atgacagttg aaggcctatc tgcctctgaa gccttaggga cattggggtt aactatggaa
     7381 aattttagcc taatgcatgc tttacctggg atgctgtcag aagcagtagg cattggtacc
     7441 ctttttcaaa ctatttcagg tgcaagtgga ctggtagcag ctgggattcg ctacggatat
     7501 gccagagaag tgtcaatagt aaacagaaat atctcccaaa tggctttgca agtttggaga
     7561 ccttgggact attatgatat tctttttcca ggggtacaaa catttgccca ctacttaaat
     7621 gttcttgatc attgggcaag cagcctgatc catactgtga gcagatatgt atgggatgct
     7681 attttgcatg aagggagaca tcaaattggc catgccagta gagaattgat gattaggggg
     7741 actaatcatt tccaagatct catggctaga ttgatagaaa atagcagatg ggtgctgact
     7801 acaggcccta gcaatatcta ttcacatctt gaatcatatt acagagattt gccagggatt
     7861 agtcctccac aagccagaga tctgtacaga agacttcagg aaaaaatacc tgacagat
//
