LOCUS       sequence-129215-         850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBAD HochAld1-20- gfpmut2- ssrA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Jakobson CM, Kim EY, Slininger MF, Chien A, Tullman-Ercek D
  TITLE     Localization of Proteins to the 1,2-Propanediol Utilization 
            Microcompartment by Non-native Signal Sequences Is Mediated by a 
            Common Hydrophobic Motif.
  JOURNAL   J Biol Chem. 2015 Aug 17. pii: jbc.M115.651919.
  PUBMED    26283792
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 17. pii: jbc.M115.651919."
COMMENT     Sequence Label: CMJ131
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(53..763)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             complement(53..763)
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(93..113)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     708..736
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     RBS             834..845
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 cgccaagctt ttaagctgct aaagcgtagt tttcgtcgtt tgctgcgtcg actttgtata
       61 gttcatccat gccatgtgta atcccagcag ctgttacaaa ctcaagaagg accatgtggt
      121 ctctcttttc gttgggatct ttcgaaaggg cagattgtgt ggacaggtaa tggttgtctg
      181 gtaaaaggac agggccatcg ccaattggag tattttgttg ataatggtct gctagttgaa
      241 cgcttccatc ttcaatgttg tgtctaattt tgaagttaac tttgattcca ttcttttgtt
      301 tgtctgccat gatgtataca ttgtgtgagt tatagttgta ttccaatttg tgtccaagaa
      361 tgtttccatc ttctttaaaa tcaatacctt ttaactcgat tctattaaca agggtatcac
      421 cttcaaactt gacttcagca cgtgtcttgt agttcccgtc atctttgaaa aatatagttc
      481 tttcctgtac ataaccttcg ggcatggcac tcttgaaaaa gtcatgctgt ttcatatgat
      541 ctgggtatct cgcaaagcat tgaagaccat acgcgaaagt agtgacaagt gttggccatg
      601 gaacaggtag ttttccagta gtgcaaataa atttaagggt aagttttccg tatgttgcat
      661 caccttcacc ctctccactg acagaaaatt tgtgcccatt aacatcacca tctaattcaa
      721 caagaattgg gacaactcca gtgaaaagtt cttctccttt acttctagag tcgaggcgcg
      781 cgagcacgcg ctcgacgatc tcggcgatgc gatcctcgcg cagagccatg acctttctcc
      841 tctttaagag
//
