LOCUS       sequence-129217-        7918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pUC19-TSV (linearized in LT) contains a genome of the 
            Trichodysplasia spinulosa-associated polyomaviruse (GenBank 
            NC_014361) linearized and cloned in the Large T-antigen coding 
            region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   van der Meijden E, Janssens RW, Lauber C, Bouwes Bavinck JN, 
            Gorbalenya AE, Feltkamp MC
  TITLE     Discovery of a new human polyomavirus associated with 
            trichodysplasia spinulosa in an immunocompromized patient.
  JOURNAL   PLoS Pathog. 2010 Jul 29;6(7):e1001024. doi: 
            10.1371/journal.ppat.1001024.
  PUBMED    20686659
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2010-07-29"; volume: "6(7):e1001024. doi"; pages: "
            10.1371/journal.ppat.1001024"
COMMENT     Sequence Label: pUC19-TSV (linearized in LT)
FEATURES             Location/Qualifiers
     source          1..7918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(42..58)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(42..58)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(55..77)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    66..82
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(90..96,97..114,115..120))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    135..156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(273..290)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(444..1032)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(524..543)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1203..1994,1995..2063))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1826..1845
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2064..2168)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2236..2254
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2292..2314)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2414..2433
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2627..2649
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2641..2658
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2642..2658
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctagagtcga cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg
       61 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      121 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      181 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      241 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      301 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      361 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      421 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      481 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      541 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      601 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      661 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      721 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      781 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      841 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
      901 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      961 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1021 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1081 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1141 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1201 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1261 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1321 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     1381 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1441 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1501 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1561 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1621 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1681 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1741 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1801 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     1861 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1921 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     1981 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     2041 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2101 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2161 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     2221 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     2281 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     2341 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     2401 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     2461 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
     2521 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     2581 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     2641 tgtaaaacga cggccagtga attcgagctc ggtacccggg gatcctctag attcatcaga
     2701 gggaatggtg gattcatggc agaagagatc tgggtcctgc atggtgtcaa acaagttgtc
     2761 ccattcttga ttgaagcttt cccaccagct ggcccaggag gggtgcccat agggaggtat
     2821 atctcttcca tcctgggttg gaacatcatg ctaaaaggaa aaactaaatt aatataggta
     2881 cataacacct gcagtttccc caccttttac ttaccccagt taaagccttg attaactctc
     2941 agatagagat tcaccaggtg aaagtcaact tctcctacta tgcaactcca agcccaaagg
     3001 gaatccaagc aatataagtc ttctccaaac catgcacagt agcatttgta acaaaagcat
     3061 tctccccaaa ctacacactt cttctggtgc atttgcttat aaacatgatg ttgaaggcct
     3121 attttgcagg ttaagcattt acactctttt aaagcttttt ctgcacactg aggccaatgt
     3181 ttaattacat tctcatcata tttttttttc ccaaaatatt ccttcaaaga tattaaattt
     3241 gcctcccaat accatgagcc tacctggcta ctgaagctag ttggaaactc ttgtcttgca
     3301 ttataaattc cttcttgcag tttttgccat aattgattta atcttgacat tttttctgga
     3361 tctcctcctt tatctggatg gtatttcaag ctcatttttt tatgattaat tttcattagt
     3421 gcaaaattcc catagcaatg ccttgggatt tgcaataaat ccataagttc aagactttct
     3481 tctctactta aaaacttatc cattttgctg aatgcaccag aagacaggta aggggagaaa
     3541 tgaagaaatg aagatgggag ctttttggag cccttttaaa gcctctgtgt gcctcaatta
     3601 attttcatca gatttggctt acataaaagg gaggaagtgc caagcctcaa gaggcctccg
     3661 gaggcctctc cctctatgtc tgtgtcaaga gggggcagga agcctcagcc tcctggtaat
     3721 actgagaaaa aacaagattt ccatagtaac cccctattag gaatttgagg aactatactt
     3781 agagtgttga cataaatagg tcattgtttg cttaagaccg ttatcctgag gaagctcgtg
     3841 cgccacctat catttgttat ggaaaatgct gtgtcagtta gtctcaaggg gatgtttgtc
     3901 atactgccac aaacacagga aggcagccaa gagacaagag gaagccagcc aaatcccgtg
     3961 attgtttttg tatattctta agatagcact tgtctaactg ctctcatcct gtccgtccgg
     4021 tctccataac aacacatcct cccgctctgt gttctcacgc ggcaaattca aattctaatg
     4081 gtaagttttt cttatttttc aggtaaaatg ggaggagtaa tcagcttatt ctttgatatt
     4141 attgaaatat caacagaatt aagtgctgct acaggatttg ctgtggatgc tgtgttagca
     4201 ggagaagcag cagctgctgt tgaagttgaa gtaatgggcc taatgacagt tgaaggccta
     4261 tctgcctctg aagccttagg gacattgggg ttaactatgg aaaattttag cctaatgcat
     4321 gctttacctg ggatgctgtc agaagcagta ggcattggta ccctttttca aactatttca
     4381 ggtgcaagtg gactggtagc agctgggatt cgctacggat atgccagaga agtgtcaata
     4441 gtaaacagaa atatctccca aatggctttg caagtttgga gaccttggga ctattatgat
     4501 attctttttc caggggtaca aacatttgcc cactacttaa atgttcttga tcattgggca
     4561 agcagcctga tccatactgt gagcagatat gtatgggatg ctattttgca tgaagggaga
     4621 catcaaattg gccatgccag tagagaattg atgattaggg ggactaatca tttccaagat
     4681 ctcatggcta gattgataga aaatagcaga tgggtgctga ctacaggccc tagcaatatc
     4741 tattcacatc ttgaatcata ttacagagat ttgccaggga ttagtcctcc acaagccaga
     4801 gatctgtaca gaagacttca ggaaaaaata cctgacagat atcaacttga ggctgccaca
     4861 gatgaatctg ctgaggtaat tgaaacctac tcagctccag gaggagcaca tcaaagagtc
     4921 tgccctgact ggatgttgcc tctggtactg ggattgtatg gtgatataac ccccactttt
     4981 ggttactatt tgagagaaga ggaaagagaa gatggccccc aaaagaaaag gagaaggatg
     5041 cgctagaaag tgccccacaa aaacatgtcc tacacccaag cctgttccaa aacttattat
     5101 gaagggcaat atagaggttc taaaccttgt tacaggccct gacagtataa caacaattga
     5161 actctatctt aacaccagga tgggacaaaa tgatgagtct aaggacaact atggttacag
     5221 tgaaaaagta actgttgcta acagcagtga ccaggacaag cctacttctg gagagatacc
     5281 aacctacagt acagctagaa ttaatctacc catgttgaat gaggacctaa cctgtaatac
     5341 tctaactatg tgggaagctg tctctgtaaa aactgaagtg gttggggtta gctctctggt
     5401 aaatgttcat atggctacta aaagaatgta tgatgacaaa ggtattggtt tcccagttga
     5461 gggaatgaat ttccatatgt ttgcagtggg tggggaaccc ctagaactcc agtttctaac
     5521 tggaaactac agaactgatt acagtgctaa tgacaaattg gttgttcccc caataaaaca
     5581 ccagagcaca caaggcctca atcctcacta caaacaaaag ctaacaaaag atggggcctt
     5641 tcctgtagag tgctggtgcc cagacccttc aaaaaatgaa aatacaagat attatggcag
     5701 ctatactggt ggtcagagta cacctccagt tttgcaattt acaaacactg tgactactgt
     5761 gctactagat gagaatggtg ttggcccact ttgtaaaggt gatggcctgt atgtgtcatg
     5821 ctgtgacata gtaggctttc tggttgggaa agatggagac atgcaataca gaggcctacc
     5881 taggtacttt aatattctat tgagaaagag aactgtaaga aatccttacc cagtttcttc
     5941 cttgttgaat aatcttttca ctggtcttat gccagctgtt cagggccagc ctatggacaa
     6001 tggactaagt acacaggttg aagaagtcag agtttatgat ggaactgaag gccttcctgg
     6061 agatccagac atggtcagat atattgacaa gtttggacaa gacaaaaccc gccccccctt
     6121 ccccgcccgg ctttattgag tcacaaacaa agtatttgct aaaaagcttt attaaatggc
     6181 ttgctttcca tgctgcaggt tttgctgtta tataaaacta ataaaggttt attgtgtttg
     6241 gaaaccagaa tcatttgttt cagagaattc ttcttcctcc tcatcctcca cctcaattac
     6301 aacagaatca agaggatctt taccctcctc cacatttaat agcaaggtag caaaattagt
     6361 atgacccatg tatttacata gaatatcttt ccaatactta acatcctcct ttattgactc
     6421 tgaaaacatg gtaattggac agtaccaaac caacaacaaa gcaatagtga ctccatcctg
     6481 tagtattctt tctgttaata agtagtcact aacctgaagg caagatctaa gattatgctt
     6541 tggagtaaaa ttcaaaacat aagcaaacct tgtaaaaatg gtttcaggca agaggtactc
     6601 attcatagtc acaagacagg gtgggaagat ctggcttctt ttgttcacat gcttcctttc
     6661 taaatttact ttaacactgc catccagatg gtctcttaaa ttatctaaat tagatactcc
     6721 ctgtccaggc tgcagctttt tattaagtgc tatttgaccc ttgacatctt caaaaataac
     6781 tacaaactgg tctattgcac atccaagttc aaagggcaat ttatctgcag gacaattgac
     6841 atttaatgtt tttcctccca ggaaattcat cagagcagct gcaaaagtag ttttcccact
     6901 attgattggt cctctaaaaa ggcaatttct ttttttaggg acattttcta caattaactt
     6961 taagattttg gtgactactt catctatatt ttcaaataag caagaatacc aagcaactcc
     7021 cgccatatac tgaagaattt taatttgacc aaactcatca gtaagtttct caaacattaa
     7081 tttaaatctt tcctccagta attcttttct agtactttca attaacttta atctctgttt
     7141 tgccatcacc acatctgctg cttgttggca ggcagttttt tgagttttgc actccttaaa
     7201 cagctttgca tttttatgat gtaagctatg gtaattatag tgagctttta gagctttttt
     7261 agtgcattta gagcatatgc ttggctcttc tgcaaagtct aagtaatgag ccataattaa
     7321 caggggatca tctagcctgt tggtaactgc aaaatcagtg agaaggttcc aattaaccac
     7381 aggcttccca ttttcttcat taaactcata ttcaaatatt cctggcttgc tttcctccag
     7441 tagcttaaat ggttcactgc acatacatct gtaacattct acttgtttaa ttactgcttt
     7501 gcataacaag aaactaacag tgcagtgtgt tacacataaa ttcttaattg ctgaaactct
     7561 atgcttgcca ggagtcatta ggaataccat gctcccttcc tgaaaagagt gcctactttt
     7621 aaattcagga ttaaatttat caagcttttt atacaaatat tcagcttttt cattagttgt
     7681 atatatcaga aaagatgata aagttttatt actatagaca gcattagaaa gaaaaggccg
     7741 caacatatca ggaaaatcat tagggacaga gtcatactta gatttttttg gctttggggg
     7801 ggtggcagag aaggagctct gggtgcagcc gggggagtcc tctggtgggg cagctcttct
     7861 tcttcttgaa ttctcctcgc taaattgagt agagggtccc ggagtggggc ttggagat
//
