LOCUS       sequence-129222-         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Planchon TA, Gao L, Milkie DE, Davidson MW, Galbraith JA, Galbraith 
            CG, Betzig E
  TITLE     Rapid three-dimensional isotropic imaging of living cells using 
            Bessel beam plane illumination.
  JOURNAL   Nat Methods. 2011 May;8(5):417-23. doi: 10.1038/nmeth.1586. Epub 
            2011 Mar 4.
  PUBMED    21378978
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1586"; journalName: "Nat Methods"; date: "2011-05"; 
            volume: "8"; issue: "5"; pages: "417-23"
COMMENT     Sequence Label: tdTomato-H2B-6
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             96..473
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      486..495
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(625..643)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggactcaga tctcgagctc
       61 aagcttcgaa ttctgcagtc gacggtaccg ccaccatgcc agagccagcg aagtctgctc
      121 ccgccccgaa aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga
      181 agcgcaagcg cagccgcaag gagagctatt ccatctatgt gtacaaggtt ctgaagcagg
      241 tccaccctga caccggcatt tcgtccaagg ccatgggcat catgaattcg tttgtgaacg
      301 acattttcga gcgcatcgca ggtgaggctt cccgcctggc gcattacaac aagcgctcga
      361 ccatcacctc cagggagatc cagacggccg tgcgcctgct gctgcctggg gagttggcca
      421 agcacgccgt gtccgagggt actaaggcca tcaccaagta caccagcgct aaggatccac
      481 cggtcgccac catggtgagc aagggcgagg aggtcatcaa agagttcatg cgcttcaagg
      541 tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc
      601 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
      661 tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc
      721 ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg
      781 tgatgaactt cgaggacggc ggtctgg
//
