LOCUS       sequence-129226-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: N/A, Emission: N/A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  TITLE     Davidson plasmids
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PDHA1-N-10
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(134..679)
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     regulatory      676..685
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
       61 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag
      121 tcgcggccgc ttaacccagc ccaggcttgc ccagtctgtg gccctcgtgg gccagcagcc
      181 actctttcac ggcgagcccg ccctcgtagc cccccacgtc caggtcgccc tgcaccaccc
      241 ggtggcaggg gatcagaatg ggcacgggat ttccgctcag ggcggttttc acggcggcgg
      301 tggcggcggg attgccggcc agggcggcca ggtggctgta gctgatgacc tctccgaact
      361 tcaccacttt cagcagtttc cacagcacct ggcgggtaaa gctctcctgc tggaacactg
      421 ggtggtgcag ggctggcaca gggaactcct cgatggcctc aggctggtga aagtaggcgt
      481 tgagccaggc ggtggcctgc atcagtggct ctggtccgcc cagcacggcg gctggggcag
      541 gcacttccac ggcgtcggcg gcagatgttc ctttgcccag gaagatgata cggtgcaggc
      601 cctgttcgca cccagacagt tccagcttgc ccagagggct atccagggtg gtgcgcttca
      661 tttcgcagtc tttgtccatg gtggcgaccg gtggatcccc tccggagcta ctgactgact
      721 taaacttgat ccactgattg gcaccacgaa cttcaaaagg tgggtcgctg gagtagatgt
      781 ggtagcccag ctcttccaaa ggtggctc
//
