LOCUS       sequence-129231-         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Module composed of 3 transcriptional units: Luciferase and Renilla 
            proteins, and the Tomato bushy stunt virus (TBSV) silencing 
            suppressor P19 plant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB3
            Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos...
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      50..302
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(337..355)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agggcggaaa gatcgccgtg taatgagctt ggaatggatc ttcgatcccg atcgttcaaa
       61 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga cgattatcat
      121 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
      181 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
      241 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
      301 tcgggaattg ccaagctaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
      361 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      421 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      481 gacaataacc ctgataaatg cttcaataat gggaccgact cgcgctgtca ggagagcgaa
      541 atgattgatg aagaacaatg gtggatgaag aacaaagaag gagggagctt ttgttcaaga
      601 tgaacaaaga acaatagtgg atgaagaaca aagtgaaaaa aataaaaa
//
