LOCUS       sequence-129234-        1125 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C9orf114 in mammalian cells with GFP reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES,
            Sabatini DM
  TITLE     Identification and characterization of essential genes in the human 
            genome.
  JOURNAL   Science. 2015 Oct 15. pii: aac7041.
  PUBMED    26472758
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Oct 15. pii: aac7041."
COMMENT     Sequence Label: pMXs-C9orf114-gfp
FEATURES             Location/Qualifiers
     source          1..1125
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      61..70
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             151..162
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aggctgccga ccccgggggt ggaccatcct ctagactgcc ggatctagct agttaattaa
       61 gccaccatgg cggagcgcgg caggaagcgg ccgtgcggcc cgggtgaaca cggccaaagg
      121 attgagtggc gaaaatggaa gcaacagaag aaagaggaga aaaaaaaatg gaaggatctc
      181 aagctgatga aaaaactgga gcggcagcgg gcacaggagg aacaggcaaa gcgcctggaa
      241 gaggaggagg cagcggcaga gaaggaggac cgcgggcggc cctacacact gagcgtagcc
      301 ctgccgggct ccatcctgga caatgctcag tcgccggagc ttcgcaccta cttggccggt
      361 cagattgcca gagcctgtgc catcttctgt gtggatgaga tcgtggtgtt tgatgaggag
      421 ggccaggatg ccaagactgt ggagggggaa ttcacaggag ttgggaagaa ggggcaggcg
      481 tgcgtacagc tggcccggat cctgcagtac ctggagtgtc cacagtacct gaggaaggcg
      541 ttcttcccca agcaccagga tctacagttt gcagggctcc tgaaccccct ggacagcccc
      601 caccacatgc gtcaggatga ggaatccgag ttccgagagg gcatcgtggt ggatcggccc
      661 acccggccag gccacggctc ctttgtcaac tgtggcatga aaaaggaggt gaagattgac
      721 aagaacctgg agcccgggct tcgggtgact gtgcgactga accagcagca gcacccagac
      781 tgcaagacct accatggcaa agtggtatca tcgcaggacc ctcgcaccaa agctggtctc
      841 tactggggct acaccgtccg actggcttcc tgcctcagtg ctgtgtttgc tgaggccccc
      901 ttccaagatg ggtatgacct gaccatcggg acgtcagagc gcggctcaga tgtggcctct
      961 gcccagcttc ccaacttcag gcatgctctt gtggtgttcg ggggcctcca gggtctggaa
     1021 gctggaccgg atgctgaccc caacctggag tggctgaacc agtgtctctt tgactgtagt
     1081 catacctgtc tggcaggtag cgtaccatcg cacggaggaa gcatc
//
