LOCUS       sequence-129237-         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Module composed of 3 transcriptional units: Luciferase and Renilla 
            proteins, and the Tomato bushy stunt virus (TBSV) silencing 
            suppressor P19 plant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB3
            Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos...
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     332..350
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(385..637)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 cgaaatgatt gatgaagaac aatggtggat gaagaacaaa gaaggaggga gcttttgttc
       61 aagatgaaca aagaacaata gtggatgaag aacaaagtga aaaaaataaa aaaaaatgta
      121 tggttaaata aagagtaaag ttaccattga gactcctgac agcgcgagtc ggtcccatta
      181 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
      241 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
      301 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
      361 tcaagaatta gcttggcaat tcccgatcta gtaacataga tgacaccgcg cgcgataatt
      421 tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga
      481 ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat
      541 gcttaacgta attcaacaga aattatatga taatcgtcgc aagaccggca acaggattca
      601 atcttaagaa actttattgc caaatgtttg aacgatcggg atcgaagatc cattccaagc
      661 tcactcgctt tctttttcg
//
