LOCUS       sequence-129249-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression; in vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-HsUBB
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..249
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             22..249
                     /codon_start=1
                     /gene="Homo sapiens UBB"
                     /product="human ubiquitin B"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             250..477
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             250..477
                     /codon_start=1
                     /gene="Homo sapiens UBB"
                     /product="human ubiquitin B"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             478..705
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 ctttaagaag gagatataca tatgcagatc ttcgtgaaaa cccttaccgg caagaccatc
       61 acccttgagg tggagcccag tgacaccatc gaaaatgtga aggccaagat ccaggataag
      121 gaaggcattc cccccgacca gcagaggctc atctttgcag gcaagcagct ggaagatggc
      181 cgtactcttt ctgactacaa cattcagaag gagtcgaccc tgcacctggt cctgcgtctg
      241 agaggtggta tgcagatctt cgtgaagacc ctgaccggca agaccatcac cctggaagtg
      301 gagcccagtg acaccatcga aaatgtgaag gccaagatcc aggataaaga aggcatccct
      361 cccgaccagc agaggctcat ctttgcaggc aagcagctgg aagatggccg cactctttct
      421 gactacaaca tccagaagga gtcgaccctg cacctggtcc tgcgtctgag aggtggtatg
      481 cagatcttcg tgaagaccct gaccggcaag accatcactc tggaggtgga gcccagtgac
      541 accatcgaaa atgtgaaggc caagatccaa gataaagaag gcatcccccc cgaccagcag
      601 aggctcatct ttgcaggcaa gcagctggaa gatggccgca ctctttctga ctacaacatc
      661 cagaaagagt cgaccctgca cctggtcctg cgcctgaggg gtggctgtta attcttcagt
      721 catggcagga tccgaattcg agctccgtcg acaagctttg cggccgcact cgagcaccac
      781 caccaccacc a
//
