LOCUS       sequence-129250-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression; in vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-HsUBB
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(109..336)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(337..564)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(337..564)
                     /codon_start=1
                     /gene="Homo sapiens UBB"
                     /product="human ubiquitin B"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(565..792)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(565..792)
                     /codon_start=1
                     /gene="Homo sapiens UBB"
                     /product="human ubiquitin B"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     RBS             800..822
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(853..872)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(854..872)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct
       61 tgtcgacgga gctcgaattc ggatcctgcc atgactgaag aattaacagc cacccctcag
      121 gcgcaggacc aggtgcaggg tcgactcttt ctggatgttg tagtcagaaa gagtgcggcc
      181 atcttccagc tgcttgcctg caaagatgag cctctgctgg tcggggggga tgccttcttt
      241 atcttggatc ttggccttca cattttcgat ggtgtcactg ggctccacct ccagagtgat
      301 ggtcttgccg gtcagggtct tcacgaagat ctgcatacca cctctcagac gcaggaccag
      361 gtgcagggtc gactccttct ggatgttgta gtcagaaaga gtgcggccat cttccagctg
      421 cttgcctgca aagatgagcc tctgctggtc gggagggatg ccttctttat cctggatctt
      481 ggccttcaca ttttcgatgg tgtcactggg ctccacttcc agggtgatgg tcttgccggt
      541 cagggtcttc acgaagatct gcataccacc tctcagacgc aggaccaggt gcagggtcga
      601 ctccttctga atgttgtagt cagaaagagt acggccatct tccagctgct tgcctgcaaa
      661 gatgagcctc tgctggtcgg ggggaatgcc ttccttatcc tggatcttgg ccttcacatt
      721 ttcgatggtg tcactgggct ccacctcaag ggtgatggtc ttgccggtaa gggttttcac
      781 gaagatctgc atatgtatat ctccttctta aagttaaaca aaattatttc tagagggaaa
      841 ccgttgtggt ctccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgggcagc
      901 gttgggtcct gtccacgggt gcgcatgatc gtgctcctgt cgttgaggac ctggctaggc
      961 tggcggggtt gccttactgg ttagcagaat gaatcaccga t
//
