LOCUS       sequence-129254-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression; in vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-MmUBB(Y305P)
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(76..303)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(304..531)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(532..759)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 ttttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcggtacct taaggaccac ccctcagacg gaggaccagg tgcagggtcg actctttctg
      121 gatgttgtaa tcagagagag tgcggccatc ttccagctgc ttgccggcaa agatcagcct
      181 ctgctggtca ggggggatgc cctctttatc ctggatcttg gccttcacat tctcgatggt
      241 gtcactgggc tccacctcca gggtgatggt cttgccggtc agggtcttca cgaagatctg
      301 catgccacct ctcaggcgaa ggaccaggtg cagggttgac tccttctgga tgttgtaatc
      361 agagagggtg cggccatctt ccagctgctt gccggcaaag atcagcctct gctggtcagg
      421 ggggatgccc tctttatcct ggatcttggc cttcacgttc tcgatggtgt cactgggctc
      481 cacctccagg gtgatggtct tgccagtcag ggtcttcacg aagatctgca tgccacccct
      541 cagacggagg accaggtgca gggttgactc cttctggatg ttgtaatcag agagggtgcg
      601 gccatcttcc agctgcttgc cggcaaagat cagcctctgc tggtcagggg ggatgccctc
      661 tttatcctgg atcttggcct tcacattctc gatggtgtca ctgggctcca cctccagggt
      721 gatggtcttg ccggtcaggg tcttcacgaa gatctgcatg ccacccctca gacggaggac
      781 caggtgcagg gttgactctt tctggatgtt gtaatcagag agggtgcggc catcttccag
      841 ctgcttgccg gcaaagatca gcctctgctg gtcaggggga tgccctcttt atcctggatc
      901 ttggcc
//
