LOCUS       sequence-129262-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a membrane bound eGFP, under the UbC promoter. This 
            promoter expresses transgenes in neurons at higher levels than 
            plasmids with the CMV or beta-actin-CMV promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  TITLE     pAAV-UbC-eGFP-F
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-UbC-eGFP-F
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(13..15,16..18,19..729)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(319..338)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggatccaagc ttatggtgtc caagggcgag gaactgttca ccggcgtggt gcccatcctg
       61 gtggaactgg atggcgacgt gaacggccac aagttcagcg tgtccggcga gggcgaaggc
      121 gacgccacat atggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      181 ccttggccta ccctcgtgac cacactgacc tacggcgtgc agtgcttcag cagatacccc
      241 gaccatatga agcagcacga cttcttcaag agcgccatgc ccgagggcta cgtgcaggaa
      301 cggaccatct tctttaagga cgacggcaac tacaagacca gggccgaagt gaagttcgag
      361 ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg acttcaaaga ggacggcaac
      421 atcctgggcc acaagctgga gtacaactac aacagccaca acgtgtacat catggccgac
      481 aagcagaaaa acggcatcaa agtgaacttc aagatccggc acaacatcga ggacggctcc
      541 gtgcagctgg ccgaccacta ccagcagaac acccccatcg gagatggccc cgtgctgctg
      601 cccgacaacc actacctgag cacacagagc gccctgagca aggaccccaa cgagaagcgg
      661 gaccacatgg tgctgctgga atttgtgacc gccgctggca tcaccctggg catggacgag
      721 ctgtacaaga gcggcctgcg gagcaagctg aacccccctg atgagtctgg ccctggctgc
      781 atgagctgca aatgcgtgct gagctaatct agactcgag
//
