LOCUS       sequence-12929-e        3911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B
  TITLE     Chaperone-based procedure to increase yields of soluble recombinant 
            proteins produced in E. coli.
  JOURNAL   BMC Biotechnol. 2007 Jun 12;7:32. doi: 10.1186/1472-6750-7-32.
  PUBMED    17565681
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-06-12"; volume: "7:32. doi"; pages: " 
            10.1186/1472-6750-7-32"
COMMENT     Sequence Label: pBB572
FEATURES             Location/Qualifiers
     source          1..3911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    32..48
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             67..78
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(1086..1108)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1194..1334
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1249..1268
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1349..1366)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1520..2108)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1600..1619)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2279..3070,3071..3139))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2902..2921
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3140..3244)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3386..3841
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3473..3492)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3683..3704
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3879..3897
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagaaaa tttatcaaaa agagtgttga cttgtgagcg gataacaatt tcacacagaa
       61 ttcattaaag aggagaaagg atccatatgc gtaactttga tttatccccg ctttaccgtt
      121 ctgctattgg atttgaccgt ttgtttaacc acttagaaaa caaccagagc cagagtaatg
      181 gcggctaccc tccgtataac gttgaactgg tagacgaaaa ccattaccgc attgctatcg
      241 ctgtggctgg ttttgctgag agcgaactgg aaattaccgc ccaggataat ctgctggtgg
      301 tgaaaggtgc tcacgccgac gaacaaaaag agcgcaccta tctgtaccag ggcatcgctg
      361 aacgcaactt tgaacgcaaa ttccagttag ctgagaacat tcatgttcgt ggtgctaacc
      421 tggtaaatgg tttgctgtat atcgatctcg aacgcgtgat tccggaagcg aaaaaaccgc
      481 gccgtatcga aatcaactaa ttccctaagg ccgcctggcg cggcctgaca tctccatgct
      541 cgccgtcagg gagcatatgc gaatcttcgg atttgcaggt acttactcgc ttcttagaag
      601 gagaaatgac tatgcgtaac tttgatttat ccccactgat gcgtcaatgg atcggttttg
      661 acaaactggc caacgcactg caaaacgccg gtgaaagcca gagcttcccg ccgtacaaca
      721 ttgagaaaag cgacgataac cactaccgca ttacccttgc gctggcaggt ttccgtcagg
      781 aagatttaga gattcaactg gaaggtacgc gcctgagcgt aaaaggcacg ccggagcagc
      841 caaaagaaga gaaaaaatgg ctgcatcaag ggcttatgaa tcagccattt agcctgagct
      901 ttacgctggc tgaaaatatg gaagtctctg gcgcaacctt cgtaaacggt ttactgcata
      961 ttgatttaat tcgtaatgag cctgaaccca tcgcagcgca gcgtatcgct atcagcgaac
     1021 gtcccgcgtt aaatagctaa ctagctctag agctgcctcg cgcgtttcgg tgatgacggt
     1081 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     1141 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     1201 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
     1261 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     1321 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1381 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1441 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1501 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1561 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1621 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1681 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1741 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1801 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1861 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1921 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     1981 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2041 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2101 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2161 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2221 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2281 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2341 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2401 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2461 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2521 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2581 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2641 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2701 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2761 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2821 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2881 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2941 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3001 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3061 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3121 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3181 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3241 cgcgcacatt tccccgaaaa gtgccacctc aggattctgg cgtaccgttc ctgtctaaaa
     3301 tccctttaat cggcctcctg tttagctccc gctctgattc taacgaggaa agcacgttat
     3361 acgtgctcgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3421 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3481 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3541 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3601 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3661 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3721 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     3781 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     3841 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     3901 ttcgtcttca c
//
