LOCUS       sequence-129299-         402 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-HISNDHFR
FEATURES             Location/Qualifiers
     source          1..402
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..51
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cacagcagcg gatctggtgg tggtggtggt gaaaacctgt acttccaggg atccatcagt
       61 ctgattgcgg cgctagcggt agatcgcgtt atcggcatgg aaaacgccat gccatggaac
      121 ctgcctgccg atctcgcctg gtttaaacgc aacaccttaa ataaacccgt gattatgggg
      181 cgccatacct gggaatcaat cggtaggcct ttgcccggcc gcaaaaatat tatcctcagc
      241 agtcaacccg ggaccgatga tcgggttacc tgggttaaat cggtcgacga agccatcgcg
      301 gcgtgtggtg acgtaccaga aatcatggtg attggcggcg gacgcgttta tgaacagttc
      361 ttgccaaaag cgcaaaaact gtatctgacg catatcgatg ca
//
