LOCUS       sequence-129305-         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-STRNDHFR
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(417..437)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(459..482)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     RBS             499..521
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(558..577)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(559..577)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcgctgaata ccgattccca gtcatccggc tcgtaatccg gaaaatgggt gtcgccttcc
       61 acttctgcat cgatatgcgt cagatacagt ttttgcgctt ttggcaagaa ctgttcataa
      121 acgcgtccgc cgccaatcac catgatttct ggtacgtcac cacacgccgc gatggcttcg
      181 tcgaccgatt taacccaggt aacccgatca tcggtcccgg gttgactgct gaggataata
      241 tttttgcggc cgggcaaagg cctaccgatt gattcccagg tatggcgccc cataatcacg
      301 ggtttattta aggtgttgcg tttaaaccag gcgagatcgg caggcaggtt ccatggcatg
      361 gcgttttcca tgccgataac gcgatctacc gctagcgccg caatcagact gatggatccc
      421 tggaagtaca ggttttcacc accaccacca ccagatcctt tctcgaactg cgggtggctc
      481 cagcttgcca tatgtatatc tccttcttaa agttaaacaa aattatttcc ggagccggag
      541 ggaaaccgtt gtggtctccc tatagtgagt cgtattaatt tcgcgggatc gatgcatggt
      601 gccaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga tttt
//
