LOCUS       sequence-129319-        1142 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_892_Nhe2_EcR-to-HLH4C_RB
FEATURES             Location/Qualifiers
     source          1..1142
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..48
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(479..503)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      694..703
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(766..784)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 acatttctct atcgataggt accacaagtt tgtacaaaaa agcaggcttc ctgcatcaaa
       61 aacacctgcg cccaggtcca ttattcataa aaaaacacct gcgccaacag agactactcg
      121 cgaatccgaa gacccaaacc gaatattact atcactcatt cgtaaaatca aagttcaccc
      181 caagaatcga ctcgattcaa gtcactaaca aggttcttac aggccttcaa aaattagact
      241 catggattat gggtagagcc gttgaacact tgaatgagtg tcttaatagg gtggccacgt
      301 tcgtaaaaac acctgcgcct attaggggac ggtaccctgg aagaagcttt cagtgcaaag
      361 gacgagttaa tagtttcaat acccggtcaa taataagcac cgacacgaaa caattataat
      421 gattatacgt tcttggtctc tgttaacctt aatccagata ataggtcgca aattaaagac
      481 ccagctttct tgtacaaagt ggtagatctg cgatcgagct cgcccgggga tcgagcgcag
      541 cggtataaaa gggcgcgggg tggctgagag catcagttgt gaatgaatgt tcgagccgag
      601 cagacgtgcc gctgccttcg ttaatatcct ttgaataagc caactttgaa tcacaagacg
      661 cataccaaac ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata
      721 aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat
      781 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
      841 gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg
      901 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
      961 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
     1021 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc
     1081 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa
     1141 aa
//
