LOCUS       sequence-129320-        1137 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_892_Nhe2_EcR-to-HLH4C_RB
FEATURES             Location/Qualifiers
     source          1..1137
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    235..259
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(690..714)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    728..819
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(760..779)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    833..881
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgg tggctttacc aacagtaccg
       61 gaatgccgtt tggtatgcgt cttgtgattc aaagttggct tattcaaagg atattaacga
      121 aggcagcggc acgtctgctc ggctcgaaca ttcattcaca actgatgctc tcagccaccc
      181 cgcgcccttt tataccgctg cgctcgatcc ccgggcgagc tcgatcgcag atctaccact
      241 ttgtacaaga aagctgggtc tttaatttgc gacctattat ctggattaag gttaacagag
      301 accaagaacg tataatcatt ataattgttt cgtgtcggtg cttattattg accgggtatt
      361 gaaactatta actcgtcctt tgcactgaaa gcttcttcca gggtaccgtc ccctaatagg
      421 cgcaggtgtt tttacgaacg tggccaccct attaagacac tcattcaagt gttcaacggc
      481 tctacccata atccatgagt ctaatttttg aaggcctgta agaaccttgt tagtgacttg
      541 aatcgagtcg attcttgggg tgaactttga ttttacgaat gagtgatagt aatattcggt
      601 ttgggtcttc ggattcgcga gtagtctctg ttggcgcagg tgttttttta tgaataatgg
      661 acctgggcgc aggtgttttt gatgcaggaa gcctgctttt ttgtacaaac ttgtggtacc
      721 tatcgataga gaaatgttct ggcacctgca cttgcactgg ggacagccta ttttgctagt
      781 ttgttttgtt tcgttttgtt ttgatggaga gcgtatgtta gtactatcga ttcacacaaa
      841 aaaccaacac acagatgtaa tgaaaataaa gatattttat tgcggccgct ccaagtacct
      901 cccgtacctt aatattactt acttatcatg gtagcttggg ctggcgtaat agcgaagagg
      961 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg caaattgtaa
     1021 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     1081 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggt
//
