LOCUS       sequence-129322-         613 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-STRHISNDHFR
FEATURES             Location/Qualifiers
     source          1..613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(463..483)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(511..528)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(538..561)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     RBS             578..600
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttcgaaacaa tagctatgcg agttctgcgc atcagcatcg tggaattcgc tgaataccga
       61 ttcccagtca tccggctcgt aatccggaaa atgggtgtcg ccttccactt ctgcatcgat
      121 atgcgtcaga tacagttttt gcgcttttgg caagaactgt tcataaacgc gtccgccgcc
      181 aatcaccatg atttctggta cgtcaccaca cgccgcgatg gcttcgtcga ccgatttaac
      241 ccaggtaacc cgatcatcgg tcccgggttg actgctgagg ataatatttt tgcggccggg
      301 caaaggccta ccgattgatt cccaggtatg gcgccccata atcacgggtt tatttaaggt
      361 gttgcgttta aaccaggcga gatcggcagg caggttccat ggcatggcgt tttccatgcc
      421 gataacgcga tctaccgcta gcgccgcaat cagactgatg gatccctgga agtacaggtt
      481 ttcaccacca ccaccaccag atccgctgct gtgatgatga tgatgatggc tagatccttt
      541 ctcgaactgc gggtggctcc agcttgccat atgtatatct ccttcttaaa gttaaacaaa
      601 attatttccg gag
//
