LOCUS       sequence-129323-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-FLAGHISNDHFR
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(483..503)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(531..548)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(558..581)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             595..617
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(654..673)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(655..673)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccggcacgac gctcgaggat ttcgaaacaa tagctatgcg agttctgcgc atcagcatcg
       61 tggaattcgc tgaataccga ttcccagtca tccggctcgt aatccggaaa atgggtgtcg
      121 ccttccactt ctgcatcgat atgcgtcaga tacagttttt gcgcttttgg caagaactgt
      181 tcataaacgc gtccgccgcc aatcaccatg atttctggta cgtcaccaca cgccgcgatg
      241 gcttcgtcga ccgatttaac ccaggtaacc cgatcatcgg tcccgggttg actgctgagg
      301 ataatatttt tgcggccggg caaaggccta ccgattgatt cccaggtatg gcgccccata
      361 atcacgggtt tatttaaggt gttgcgttta aaccaggcga gatcggcagg caggttccat
      421 ggcatggcgt tttccatgcc gataacgcga tctaccgcta gcgccgcaat cagactgatg
      481 gatccctgga agtacaggtt ttcaccacca ccaccaccag atccgctgct gtgatgatga
      541 tgatgatggc tagatccctt gtcatcatcg tccttgtagt ctcccatatg tatatctcct
      601 tcttaaagtt aaacaaaatt atttccggag ccggagggaa accgttgtgg tctccctata
      661 gtgagtcgta ttaatttcgc gggatcgatg catggtgcca acacctacat ctgtattaac
      721 gaagcgctgg cattgaccct gagtgat
//
